N-Cadherin Antikörper (AA 802-819)

Produktnummer ABIN968250

Produktdetails anti-N-Cadherin Antikörper

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Bindungsspezifität: AA 802-819

Reaktivität: Human, Maus, Ratte, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser N-Cadherin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Kreuzreaktivität: Human, Ratte (Rattus), Huhn

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse N-Cadherin aa. 802-819

Klon: 32-N

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389, ABIN968535

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-N-Cadherin Antikörper (ABIN968250)

Izawa, Nishizawa, Ohtakara, Inagaki: "Densin-180 interacts with delta-catenin/neural plakophilin-related armadillo repeat protein at synapses." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 5345-50, (2002) (PubMed).

Nürnberger, Bacallao, Phillips: "Inversin forms a complex with catenins and N-cadherin in polarized epithelial cells." in: Molecular biology of the cell, Vol. 13, Issue 9, pp. 3096-106, (2002) (PubMed).

Bhowmick, Ghiassi, Bakin, Aakre, Lundquist, Engel, Arteaga, Moses: "Transforming growth factor-beta1 mediates epithelial to mesenchymal transdifferentiation through a RhoA-dependent mechanism." in: Molecular biology of the cell, Vol. 12, Issue 1, pp. 27-36, (2001) (PubMed).

Cowin: "Unraveling the cytoplasmic interactions of the cadherin superfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 23, pp. 10759-61, (1994) (PubMed).

Miyatani, Shimamura, Hatta, Nagafuchi, Nose, Matsunaga, Hatta, Takeichi: "Neural cadherin: role in selective cell-cell adhesion." in: Science (New York, N.Y.), Vol. 245, Issue 4918, pp. 631-5, (1989) (PubMed).

Details zu N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Andere Bezeichnung: N-Cadherin (CDH2 Produkte)

Hintergrund: Cadherins are a family of Ca2+-dependent intercellular adhesion molecules that play a central role in controlling morphogenetic movements during development. Their function is regulated by association with the actin cytoskeleton by a complex of cytoplasmic proteins called the catenins (alpha, beta, gamma). Members of the cadherin family include P-cadherin, E-cadherin (uvomorulin), N-cadherin (neural cadherin), R-cadherin, cadherin 5, L-CAM, and EP-cadherin. N-cadherin mRNA is found at elevated levels in brain and heart and at a much lower level in liver. Mechanisms such as mRNA expression, cytokine modulation, and protease-mediated turnover modulate N-cadherin protein levels during development. In addition, N-cadherin function is indirectly regulated by endogenous kinases and phosphatases. Tyrosine phosphorylation of beta-catenin complexed with N-cadherin results in dissociation of N-cadherin from actin. However, N-cadherin also interacts with a PTP1B-like phosphatase that dephosphorylates beta-catenin and promotes N-cadherin/actin association. Thus, N-cadherin is an integral adhesion molecule whose function is regulated by protein-protein interactions and phosphorylation/dephosphorylation events.

Molekulargewicht: 130 kDa

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane