FYB Antikörper (AA 673-783)

Produktnummer ABIN968265

Produktdetails anti-FYB Antikörper

Target: FYB (FYN-Binding Protein (FYB))

Bindungsspezifität: AA 673-783

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FYB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human FYB aa. 673-783

Klon: 5-FYB

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-FYB Antikörper (ABIN968265)

Geng, Pfister, Kraeft, Rudd: "Adaptor FYB (Fyn-binding protein) regulates integrin-mediated adhesion and mediator release: differential involvement of the FYB SH3 domain." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 20, pp. 11527-32, (2001) (PubMed).

Oda, Ikeda, Ochs, Druker, Ozaki, Handa, Ariga, Sakiyama, Witte, Wahl: "Rapid tyrosine phosphorylation and activation of Bruton's tyrosine/Tec kinases in platelets induced by collagen binding or CD32 cross-linking." in: Blood, Vol. 95, Issue 5, pp. 1663-70, (2000) (PubMed).

da Silva, Li, de Vera, Canto, Findell, Rudd: "Cloning of a novel T-cell protein FYB that binds FYN and SH2-domain-containing leukocyte protein 76 and modulates interleukin 2 production." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 14, pp. 7493-8, (1997) (PubMed).

Musci, Hendricks-Taylor, Motto, Paskind, Kamens, Turck, Koretzky: "Molecular cloning of SLAP-130, an SLP-76-associated substrate of the T cell antigen receptor-stimulated protein tyrosine kinases." in: The Journal of biological chemistry, Vol. 272, Issue 18, pp. 11674-7, (1997) (PubMed).

Details zu FYB

Target: FYB (FYN-Binding Protein (FYB))

Andere Bezeichnung: FYB (FYB Produkte)

Synonyme: fyb antikoerper, ADAP antikoerper, PRO0823 antikoerper, SLAP-130 antikoerper, B630013F22Rik antikoerper, FYB-120/130 antikoerper, Slap130 antikoerper, p120/130 antikoerper, p120/p130 antikoerper, RGD1563421 antikoerper, FYN-binding protein antikoerper, FYN binding protein 1 antikoerper, FYN binding protein antikoerper, fyb antikoerper, FYB1 antikoerper, Fyb antikoerper, Fyb1 antikoerper

Hintergrund: Engagement of the T cell receptor (TcR) results in the activation of multiple intracellular src protein tyrosine kinases (PTKs) including p56 [lck] and p59 [fyn]. PTK activation induces TcRzeta phosphorylation and the subsequent recruitment and activation of ZAP-70 which specifically phosphorylates SLP-76. SLP-76 interacts with multiple components of T cell signaling pathways, including Grb-2, PLC-gamma, Vav, and SLAP-130 (also known as SLP-76 Associated Phosphoprotein of 130 kDa). SLAP-130 contains multiple tyrosine based motifs, proline base type I and II SH3 domain binding motifs, lysine/glutamic acid rich nuclear localization motifs, and an SH3-like domain. It associates with the SH2 domain of SLP-76 and is phosphorylated by TcR induced PTKs. This protein has also been reported to directly interact with fyn and has been referred to as fyn binding protein (FYB). Co-transfection data has shown that a limited concentration of FYB/SLAP-130 enhances TcR-induced IL-2 production, while overexpression downregulates gene expression. Thus, FYB/SLAP-130 is viewed to be a regulatory component of the fyn/SLP-76 signaling cascade in T cells.
Synonyms: Fyn Binding Protein, SLAP-130

Molekulargewicht: 130 kDa

Pathways: T-Zell Rezeptor Signalweg, Protein targeting to Nucleus