RAD50 Antikörper (AA 672-786)

Produktnummer ABIN968313

Dieses Anti-RAD50-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von RAD50 in WB und IF. Geeignet für Human. Dieses Primary Antibody wurde in 5+ Publikationen zitiert.

Kurzübersicht für RAD50 Antikörper (AA 672-786) (ABIN968313)

Target: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RAD50 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 13-RAD50

Produktdetails anti-RAD50 Antikörper

Bindungsspezifität: AA 672-786

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human RAD50 aa. 672-786

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-RAD50 Antikörper (ABIN968313)

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Huber, Yang, Sarkisian, Master, Deng, Chodosh: "Impaired DNA damage response in cells expressing an exon 11-deleted murine Brca1 variant that localizes to nuclear foci." in: Molecular and cellular biology, Vol. 21, Issue 12, pp. 4005-15, (2001) (PubMed).

Ohta, Nicolas, Furuse, Nabetani, Ogawa, Shibata: "Mutations in the MRE11, RAD50, XRS2, and MRE2 genes alter chromatin configuration at meiotic DNA double-stranded break sites in premeiotic and meiotic cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 2, pp. 646-51, (1998) (PubMed).

Trujillo, Yuan, Lee, Sung: "Nuclease activities in a complex of human recombination and DNA repair factors Rad50, Mre11, and p95." in: The Journal of biological chemistry, Vol. 273, Issue 34, pp. 21447-50, (1998) (PubMed).

Dolganov, Maser, Novikov, Tosto, Chong, Bressan, Petrini: "Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair." in: Molecular and cellular biology, Vol. 16, Issue 9, pp. 4832-41, (1996) (PubMed).

Details zu RAD50

Target: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Andere Bezeichnung: Rad50

Hintergrund: DNA double-strand breaks (DSBs) are generated during intrinsic eukaryotic DNA recombination events such as assembly of antigen receptor genes, meiotic and miotic recombination. DNA DSB repair proteins are also required to repair breaks induced by extrinsic factors such as ionizing radiation and mutagenic chemicals. Originally identified in S. cerevisiae, Rad50 is one of a group of genes, designated as the Rad52 epistasis group, whose products mediate DSB repair. Many of these genes, including Rad50, are conserved in humans and are thought to have a similar function to their S. cerevisiae counterparts. In yeast, a multiprotein complex of Rad50, MRE11, and XRS2 has been implicated in the nucleocytic processing of DSBs. In humans, Rad50 and MRE11 complex with up to three additional proteins (95 kDa, 200 kDa, and 350 kDa). The 95 kDa species is thought to be human XRS2, although a separate report has identified it as Nibrin, the product of the gene mutated in Nijmegen breakage syndrome. The Rad50-MRE11-p95 complex possess endonuclease and 3' to 5' exonuclease activity. Thus, human Rad50 functions in a multiprotein complex to mediate the repair of DSBs in the human genome. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 154 kDa

Pathways: DNA Reparatur, Protein targeting to Nucleus