ARNT Antikörper (AA 461-574)

Produktnummer ABIN968355

Produktdetails anti-ARNT Antikörper

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Bindungsspezifität: AA 461-574

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ARNT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Kreuzreaktivität: Maus, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human HIF-1beta/ARNT1 aa. 461-574

Klon: 29-HIF

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-ARNT Antikörper (ABIN968355)

Ambrosini, Nath, Sierra-Honigmann, Flores-Riveros: "Transcriptional activation of the human leptin gene in response to hypoxia. Involvement of hypoxia-inducible factor 1." in: The Journal of biological chemistry, Vol. 277, Issue 37, pp. 34601-9, (2002) (PubMed).

Jiang, Jiang, Zheng, Lu, Hunter, Vogt: "Phosphatidylinositol 3-kinase signaling controls levels of hypoxia-inducible factor 1." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 12, Issue 7, pp. 363-9, (2001) (PubMed).

Suzuki, Tomida, Tsuruo: "Dephosphorylated hypoxia-inducible factor 1alpha as a mediator of p53-dependent apoptosis during hypoxia." in: Oncogene, Vol. 20, Issue 41, pp. 5779-88, (2001) (PubMed).

Maltepe, Schmidt, Baunoch, Bradfield, Simon: "Abnormal angiogenesis and responses to glucose and oxygen deprivation in mice lacking the protein ARNT." in: Nature, Vol. 386, Issue 6623, pp. 403-7, (1997) (PubMed).

Drutel, Kathmann, Heron, Schwartz, Arrang: "Cloning and selective expression in brain and kidney of ARNT2 homologous to the Ah receptor nuclear translocator (ARNT)." in: Biochemical and biophysical research communications, Vol. 225, Issue 2, pp. 333-9, (1996) (PubMed).

Details zu ARNT

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Andere Bezeichnung: HIF-1beta (ARNT Produkte)

Synonyme: HIF-1-beta antikoerper, HIF-1beta antikoerper, HIF1-beta antikoerper, HIF1B antikoerper, HIF1BETA antikoerper, TANGO antikoerper, bHLHe2 antikoerper, D3Ertd557e antikoerper, Drnt antikoerper, ESTM42 antikoerper, Hif1b antikoerper, W08714 antikoerper, mKIAA4051 antikoerper, Arnt1 antikoerper, ARNT antikoerper, zgc:136664 antikoerper, arnt1 antikoerper, hif1b antikoerper, tango antikoerper, xArnt antikoerper, hif1beta antikoerper, hif-1beta antikoerper, arnt.2 antikoerper, MGC68805 antikoerper, aryl hydrocarbon receptor nuclear translocator antikoerper, aryl hydrocarbon receptor nuclear translocator S homeolog antikoerper, ARNT antikoerper, Arnt antikoerper, arnt antikoerper, arnt.S antikoerper

Hintergrund: The Ah-receptor (AHR) is a ligand activated transcription factor that mediates the biological effects of agonists. AHR dimerizes with a structurally related protein known as ARNT (arylhydrocarbon-receptor nuclear transducer). This heterodimer binds enhancer elements and induces the expression of target genes, specifically those involved in the metabolism of xenobiotics. ARNT1 and ARNT2 are members of the basic-helix-loop-helix-PAS family of heterodimeric transcription factors, which also includes AHR, hypoxia-inducible factor-1alpha (HIF-1alpha), and the Drosophila single-minded protein (Sim). While ARNT2 expression is limited to brain and kidney, ARNT1 exhibits ubiquitous expression. A targeted disruption of the Arnt locus in the mouse yields embryonic stem cells that fail to activate genes that normally respond to low oxygen tension. Arnt -/- embryos do not survive and show defective angiogenesis of the yolk sac and branchial arches, stunted development, and wasting. Thus, in addition to its regulation of xenobiotic metabolism genes, ARNT is thought to induce developmental gene expression resulting in vascularization of the developing embryo.

Molekulargewicht: 95 kDa

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effekt