CDH15 Antikörper (AA 253-366)

Produktnummer ABIN968363

Produktdetails anti-CDH15 Antikörper

Target: CDH15 (M-Cadherin (CDH15))

Bindungsspezifität: AA 253-366

Reaktivität: Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CDH15 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Kreuzreaktivität: Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse M-Cadherin aa. 253-366

Klon: 5-M

Isotyp: IgG2a

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-CDH15 Antikörper (ABIN968363)

Kang, Feinleib, Knox, Ketteringham, Krauss: "Promyogenic members of the Ig and cadherin families associate to positively regulate differentiation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 7, pp. 3989-94, (2003) (PubMed).

Kaufmann, Kirsch, Irintchev, Wernig, Starzinski-Powitz: "The M-cadherin catenin complex interacts with microtubules in skeletal muscle cells: implications for the fusion of myoblasts." in: Journal of cell science, Vol. 112 ( Pt 1), pp. 55-68, (1999) (PubMed).

Shimoyama, Shibata, Kitajima, Hirohashi: "Molecular cloning and characterization of a novel human classic cadherin homologous with mouse muscle cadherin." in: The Journal of biological chemistry, Vol. 273, Issue 16, pp. 10011-8, (1998) (PubMed).

Kuch, Winnekendonk, Butz, Unvericht, Kemler, Starzinski-Powitz: "M-cadherin-mediated cell adhesion and complex formation with the catenins in myogenic mouse cells." in: Experimental cell research, Vol. 232, Issue 2, pp. 331-8, (1997) (PubMed).

Donalies, Cramer, Ringwald, Starzinski-Powitz: "Expression of M-cadherin, a member of the cadherin multigene family, correlates with differentiation of skeletal muscle cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 18, pp. 8024-8, (1991) (PubMed).

Details zu CDH15

Target: CDH15 (M-Cadherin (CDH15))

Andere Bezeichnung: M-Cadherin (CDH15 Produkte)

Synonyme: AI323380 antikoerper, Cdh14 antikoerper, Mcad antikoerper, CDH14 antikoerper, CDH3 antikoerper, CDHM antikoerper, MCAD antikoerper, MRD3 antikoerper, cadherin 15 antikoerper, Cdh15 antikoerper, CDH15 antikoerper

Hintergrund: Cadherins are a family of transmembrane glycoproteins involved in the Ca2+-dependent cell-cell adhesion that occurs in many tissues. Members of this family include P-Cadherin, E-Cadherin (uvomorulin), N-Cadherin, R-Cadherin, Cadherin-5, L-CAM, and EP-Cadherin. These proteins are similar in their domain structure (45-74% amino acid conservation), Ca2+ and protease sensitivity, and molecular weight. However, cadherins have distinct tissue expression patterns and immunological reactivities. M (muscle)-Cadherin, another member of the Cadherin family, was discovered in myogenic mouse cells where it is present at low levels in myoblasts. It is expressed in prenatal and adult skeletal muscle and plays a role in skeletal muscle cell differentiation, particularly the fusion of myoblasts into myotubes. It is upregulated upon induction of myotube formation. M-Cadherin also forms complexes with the catenins in skeletal muscle cells, which then interact with the cytoskeleton. Therefore, it is thought that the M-Cadherin-cytoskeleton interaction may play a role in aligning myoblasts during fusion. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 130 kDa