RAD9A Antikörper (AA 264-370)

Produktnummer ABIN968473

Produktdetails anti-RAD9A Antikörper

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Bindungsspezifität: AA 264-370

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RAD9A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Kreuzreaktivität: Maus, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human hRAD9 aa. 264-370

Klon: 56-hRad9

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968536, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-RAD9A Antikörper (ABIN968473)

Yoshida, Komatsu, Wang, Kufe: "c-Abl tyrosine kinase regulates the human Rad9 checkpoint protein in response to DNA damage." in: Molecular and cellular biology, Vol. 22, Issue 10, pp. 3292-300, (2002) (PubMed).

Komatsu, Miyashita, Hang, Hopkins, Zheng, Cuddeback, Yamada, Lieberman, Wang: "Human homologue of S. pombe Rad9 interacts with BCL-2/BCL-xL and promotes apoptosis." in: Nature cell biology, Vol. 2, Issue 1, pp. 1-6, (2000) (PubMed).

Volkmer, Karnitz: "Human homologs of Schizosaccharomyces pombe rad1, hus1, and rad9 form a DNA damage-responsive protein complex." in: The Journal of biological chemistry, Vol. 274, Issue 2, pp. 567-70, (1999) (PubMed).

St Onge, Udell, Casselman, Davey: "The human G2 checkpoint control protein hRAD9 is a nuclear phosphoprotein that forms complexes with hRAD1 and hHUS1." in: Molecular biology of the cell, Vol. 10, Issue 6, pp. 1985-95, (1999) (PubMed).

Lieberman, Hopkins, Nass, Demetrick, Davey: "A human homolog of the Schizosaccharomyces pombe rad9+ checkpoint control gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 24, pp. 13890-5, (1997) (PubMed).

Details zu RAD9A

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Andere Bezeichnung: hRad9 (RAD9A Produkte)

Synonyme: RAD9 antikoerper, Rad9 antikoerper, rad9a antikoerper, MGC89062 antikoerper, XRad9 antikoerper, rad9 antikoerper, RAD9 checkpoint clamp component A antikoerper, RAD9 checkpoint clamp component B antikoerper, RAD9 checkpoint clamp component A L homeolog antikoerper, RAD9A antikoerper, Rad9a antikoerper, rad9a antikoerper, rad9b antikoerper, rad9a.L antikoerper

Hintergrund: Cell cycle checkpoints are regulatory mechanisms that prevent cell cycle progression in the presence of DNA damage or incompletely replicated DNA. Many of the genes required for cell-cycle arrest are also involved in DNA repair, apoptosis, and induction of transcription. In yeast and humans, hRAD9 plays a role in cell cycle arrest during the G2 phase before entry into mitosis. Phosphorylated hRAD9 is found in the nucleus after DNA damage, and forms DNA damage-responsive complexes with other putative checkpoint control proteins, such as hRAD1 and hHUS1. Expression of hRAD9 in S. pombe rad9::ura4+ cells restores resistance to the DNA synthesis inhibitor hydroxyurea and gamma rays. In addition, hRAD9 binds the anti-apoptotic proteins, Bcl-2 and Bcl-xL , and antisense hRAD9 RNA suppresses DNA-damage induced cell death. Thus, hRAD9 may be an important component of protein complexes that regulate cell cycle progression, as well as apoptosis, in response to DNA damage.

Molekulargewicht: 60 kDa

Pathways: Positive Regulation of Response to DNA Damage Stimulus