NPAT Antikörper (AA 681-803)

Produktnummer ABIN968484

Produktdetails anti-NPAT Antikörper

Target: NPAT (Nuclear Protein, Ataxia-Telangiectasia Locus (NPAT))

Bindungsspezifität: AA 681-803

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NPAT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human NPAT aa. 681-803

Klon: 27-NPAT

Isotyp: IgG2b

Anwendungsinformationen

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-NPAT Antikörper (ABIN968484)

Zhao, Dynlacht, Imai, Hori, Harlow: "Expression of NPAT, a novel substrate of cyclin E-CDK2, promotes S-phase entry." in: Genes & development, Vol. 12, Issue 4, pp. 456-61, (1998) (PubMed).

Details zu NPAT

Target: NPAT (Nuclear Protein, Ataxia-Telangiectasia Locus (NPAT))

Andere Bezeichnung: NPAT (NPAT Produkte)

Hintergrund: Cyclins regulate transitions between cell cycle phases by acting as regulatory subunits of the cyclin-dependent kinases (cdk). The temporal expression of cyclins is tightly regulated and plays a critical role in controlling the enzymatic activity of the cdks. Cyclin-dependent kinase 2 (Cdk2) is a member of a family of cdc2-related cell cycle protein kinases. Cdk2 is expressed earlier in the cell cycle than cdc2 and forms complexes with cyclins A, E, D1, and D3. It is not known if the D cyclins can form active complexes with Cdk2. Cyclin E-Cdk2 kinase is active in the G1 and S phases of the cell cycle and is important, as is Cyclin A-Cdk2, for the progression from G1 to S phase. One substrate for cyclin E-Cdk2 is a Nuclear Protein mapped to the Ataxia Telangiectasia locus, NPAT. This protein associates with cyclin E-Cdk2 and can be phosphorylated by Cdk2. NPAT protein levels peak at the G1/S boundary and overexpression of NPAT accelerates S phase entry, especially after coexpression of cyclin E-Cdk2. Thus, NPAT is a substrate of cyclin E-Cdk2 that may mediate G1 to S phase transition.
Synonyms: Nuclear Protein Ataxia Telangiectasia

Molekulargewicht: 210 kDa