MSH3 Antikörper (AA 136-349)

Produktnummer ABIN968513

Produktdetails anti-MSH3 Antikörper

Target: MSH3 (MutS Homolog 3 (MSH3))

Bindungsspezifität: AA 136-349

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MSH3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human MSH3 aa. 136-349

Klon: 52-MSH3

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389, ABIN968535

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-MSH3 Antikörper (ABIN968513)

Wilson, Guerrette, Fishel: "Dissociation of mismatch recognition and ATPase activity by hMSH2-hMSH3." in: The Journal of biological chemistry, Vol. 274, Issue 31, pp. 21659-64, (1999) (PubMed).

Umar, Risinger, Glaab, Tindall, Barrett, Kunkel: "Functional overlap in mismatch repair by human MSH3 and MSH6." in: Genetics, Vol. 148, Issue 4, pp. 1637-46, (1998) (PubMed).

Watanabe, Ikejima, Suzuki, Shimada: "Genomic organization and expression of the human MSH3 gene." in: Genomics, Vol. 31, Issue 3, pp. 311-8, (1997) (PubMed).

New, Liu, Crouse: "The yeast gene MSH3 defines a new class of eukaryotic MutS homologues." in: Molecular & general genetics : MGG, Vol. 239, Issue 1-2, pp. 97-108, (1993) (PubMed).

Details zu MSH3

Target: MSH3 (MutS Homolog 3 (MSH3))

Andere Bezeichnung: MSH3 (MSH3 Produkte)

Synonyme: ATMSH3 antikoerper, M7J2.90 antikoerper, M7J2_90 antikoerper, homolog of DNA mismatch repair protein MSH3 antikoerper, DUP antikoerper, MRP1 antikoerper, D13Em1 antikoerper, Rep-3 antikoerper, Rep3 antikoerper, mutS homolog 3 antikoerper, DNA mismatch repair protein MSH3 antikoerper, homolog of DNA mismatch repair protein MSH3 antikoerper, mismatch repair protein antikoerper, MSH3 antikoerper, Msh3 antikoerper

Hintergrund: Bacterial mismatch DNA repair involves the MutL, MutH, and MutS proteins, which forms a complex that mediates excision repair. Mutations in or deficiencies of any of these proteins results in a mutator phenotype that is characterized by genetic instability. Human homologs of MutS include MSH2, MSH3, and MSH6. MSH2 forms heterodimers with MSH6 (hMutSalpha) or MSH3 (hMutSbeta) that specifically bind single-mispaired nucleotides and a subset of insertion-deletion mismatches. In addition, these heterodimers have intrinsic ATPase activity that is regulated by mismatch binding. ADP-bound heterodimers bind mismatched nucleotides, while ATP-bound heterodimers do not. The role of MSH3 in genetic stability in human cells in unclear. However, MSH3 and MSH6 share roles in the control of mutation rates. Both participate in repair of replication errors containing base-base mismatches or 1-4 extra bases. The MSH3 gene is located upstream of the dihydrofolate reductase (DHFR) gene and is expressed at low levels in a variety of human tissues. Thus, MSH3 is a component of an adenosine nucleotide-regulated molecular switch whose activity is essential for classical nucleotide mismatch repair.

Molekulargewicht: 127 kDa

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response