ube3a Antikörper (AA 501-712)

Produktnummer ABIN968525

Produktdetails anti-ube3a Antikörper

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Bindungsspezifität: AA 501-712

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ube3a Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Kreuzreaktivität: Human

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse UBE3A aa. 501-712

Klon: 13-UBE3A

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968584, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-ube3a Antikörper (ABIN968525)

Nawaz, Lonard, Smith, Lev-Lehman, Tsai, Tsai, OMalley: "The Angelman syndrome-associated protein, E6-AP, is a coactivator for the nuclear hormone receptor superfamily." in: Molecular and cellular biology, Vol. 19, Issue 2, pp. 1182-9, (1999) (PubMed).

Matsuura, Sutcliffe, Fang, Galjaard, Jiang, Benton, Rommens, Beaudet: "De novo truncating mutations in E6-AP ubiquitin-protein ligase gene (UBE3A) in Angelman syndrome." in: Nature genetics, Vol. 15, Issue 1, pp. 74-7, (1997) (PubMed).

Details zu ube3a

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Andere Bezeichnung: UBE3A (ube3a Produkte)

Hintergrund: Protein ubiquitination is the end product of a multienzyme cascade that involves sequential thiol-ester bond interactions between ubiquitin and enzymes termed E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin protein ligases. UBE3A (human papilloma virus E6-associated protein, E6-AP) is an E3 ubiquitin protein ligase that is linked to the neurological disorder Angelman syndrome. It mediates the interaction of human papilloma virus types 16 and 18 E6 proteins with p53, which targets p53 to the ubiquitination-proteasome degradation pathway. The C-terminal region of UBE3A contains a hect domain, which is found in many E3 ubiquitin protein ligases, while the central region contains an E6 binding region. UBE3A is expressed in the cytoplasm and nucleus of many tissues. The interaction of UBE3A with the progesterone receptor coactivates transciptional activity of the receptor in the presence of bound hormone. Thus, UBE3A is an essential component of the ubiquitination degradation pathway and the nuclear hormone receptor signal transduction pathways. This antibody is routinely tested by western blot analysis.
Synonyms: Ubiquitin Protein Ligase 3A

Molekulargewicht: 100 kDa

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway