Dynamin Associated Protein 160 (DAP160) (AA 800-909) Antikörper

Produktnummer ABIN968598

Produktdetails

Target: Dynamin Associated Protein 160 (DAP160)

Bindungsspezifität: AA 800-909

Reaktivität: Huhn, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Applikation: Immunofluorescence (IF), Western Blotting (WB)

Kreuzreaktivität: Ratte (Rattus), Huhn

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Ese-1 aa. 800-909

Klon: 29-Intersectin

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968545, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen

Okamoto, Schoch, Südhof: "EHSH1/intersectin, a protein that contains EH and SH3 domains and binds to dynamin and SNAP-25. A protein connection between exocytosis and endocytosis?" in: The Journal of biological chemistry, Vol. 274, Issue 26, pp. 18446-54, (1999) (PubMed).

Sengar, Wang, Bishay, Cohen, Egan: "The EH and SH3 domain Ese proteins regulate endocytosis by linking to dynamin and Eps15." in: The EMBO journal, Vol. 18, Issue 5, pp. 1159-71, (1999) (PubMed).

Antigendetails

Target: Dynamin Associated Protein 160 (DAP160)

Andere Bezeichnung: Intersectin

Hintergrund: ESE-1 (epithelium-specific, EH domain and SH3 domain regulator of endocytosis) contains two N-terminal EH (Ep15 homology) domains, a central coiled-coil domain, and five C-terminal SH3 domains. ESE-1 is also known as the adaptor protein Intersectin. Intersectin constitutively associates with Eps15 to form a complex that is analogous to the yeast Pan1p-End3p complex, which is required for endocytosis and organization of the actin cytoskeleton. The EH, coiled-coil, and SH3 domains of intersectin bind epsin family proteins, SNAP25 and SNAP23, and dynamin, respectively. Thus, intersectin functions in complex with Eps15 to regulate endocytosis by linking components of the endocytic machinery.

Molekulargewicht: 140/200 kDa