Thyroid Hormone Receptor Interactor 11 (TRIP11) (AA 159-365) Antikörper

Produktnummer ABIN968639

Produktdetails

Target: Thyroid Hormone Receptor Interactor 11 (TRIP11)

Bindungsspezifität: AA 159-365

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human GMAP-210 aa. 159-365

Klon: 15-GMAP

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen

Chen, Chen, Chen, Sharp, Lee: "Thyroid hormone, T3-dependent phosphorylation and translocation of Trip230 from the Golgi complex to the nucleus." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 8, pp. 4443-8, (1999) (PubMed).

Infante, Ramos-Morales, Fedriani, Bornens, Rios: "GMAP-210, A cis-Golgi network-associated protein, is a minus end microtubule-binding protein." in: The Journal of cell biology, Vol. 145, Issue 1, pp. 83-98, (1999) (PubMed).

Chang, Chen, Chen, Chou, Chen, Ma, Yang-Feng, Leng, Tsai, OMalley, Lee: "A thyroid hormone receptor coactivator negatively regulated by the retinoblastoma protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 17, pp. 9040-5, (1997) (PubMed).

Antigendetails

Target: Thyroid Hormone Receptor Interactor 11 (TRIP11)

Andere Bezeichnung: GMAP-210 (TRIP11 Produkte)

Synonyme: ACG1A antikoerper, CEV14 antikoerper, GMAP-210 antikoerper, TRIP-11 antikoerper, TRIP230 antikoerper, cb423 antikoerper, sb:cb423 antikoerper, sb:cb721 antikoerper, wu:fb71d07 antikoerper, MGC79667 antikoerper, 2610511G22Rik antikoerper, 3110031G15Rik antikoerper, 6030460N08Rik antikoerper, AI450776 antikoerper, thyroid hormone receptor interactor 11 antikoerper, thyroid hormone receptor interactor 11 L homeolog antikoerper, TRIP11 antikoerper, trip11 antikoerper, trip11.L antikoerper, Trip11 antikoerper

Hintergrund: Trip230 was identified as a protein that interacts with retinoblastoma protein (Rb) and the thyroid hormone receptor (TR). The structure of Trip230 includes five coiled-coil segments separated by non-helical linkers, N-terminal and C-terminal leucine zipper domains, and multiple phosphorylation sites. Trip230 is ubiquitously expressed and localized to the Golgi. During cell cycle progression, a significant portion of Trip230 translocates to the nucleus. In addition, activation of TR with thyroid hormone (T3) leads to phosphorylation of Trip230, as well as Trip230 translocation from the Golgi to the nucleus. Interestingly, Trip230 has also been identified as a Golgi microtubule-associated protein of 210 kDa (GMAP-210). In vitro , GMAP-210 can bind to the minus end of alpha-tubulin and gamma-tubulin via its C-terminal region, while the N-terminal region is involved in Golgi binding. Overexpression of GMAP-210 leads to enlargement of the Golgi apparatus and alterations in the microtubule cytoskeleton. Thus, GMAP-210/Trip230 is thought to function both as a TR coactivator and as a microtubule-binding protein that anchors the Golgi to the microtubule cytoskeleton.
Synonyms: Trip230

Molekulargewicht: 210 kDa