PSIP1 Antikörper (AA 85-188)

Produktnummer ABIN968640

Produktdetails anti-PSIP1 Antikörper

Target: PSIP1 (PC4 and SFRS1 Interacting Protein 1 (PSIP1))

Bindungsspezifität: AA 85-188

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PSIP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human LEDGF aa. 85-188

Klon: 26-LEDGF

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Bioimaging:
Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.

Kommentare:

Related Products: ABIN968535, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-PSIP1 Antikörper (ABIN968640)

Cherepanov, Maertens, Proost, Devreese, Van Beeumen, Engelborghs, De Clercq, Debyser: "HIV-1 integrase forms stable tetramers and associates with LEDGF/p75 protein in human cells." in: The Journal of biological chemistry, Vol. 278, Issue 1, pp. 372-81, (2002) (PubMed).

Nakamura, Singh, Kubo, Chylack, Shinohara: "LEDGF: survival of embryonic chick retinal photoreceptor cells." in: Investigative ophthalmology & visual science, Vol. 41, Issue 5, pp. 1168-75, (2000) (PubMed).

Singh, Kimura, Chylack, Shinohara: "Lens epithelium-derived growth factor (LEDGF/p75) and p52 are derived from a single gene by alternative splicing." in: Gene, Vol. 242, Issue 1-2, pp. 265-73, (2000) (PubMed).

Ge, Si, Wolffe: "A novel transcriptional coactivator, p52, functionally interacts with the essential splicing factor ASF/SF2." in: Molecular cell, Vol. 2, Issue 6, pp. 751-9, (1999) (PubMed).

Ge, Si, Roeder: "Isolation of cDNAs encoding novel transcription coactivators p52 and p75 reveals an alternate regulatory mechanism of transcriptional activation." in: The EMBO journal, Vol. 17, Issue 22, pp. 6723-9, (1999) (PubMed).

Details zu PSIP1

Target: PSIP1 (PC4 and SFRS1 Interacting Protein 1 (PSIP1))

Andere Bezeichnung: LEDGF (PSIP1 Produkte)

Synonyme: PSIP1 antikoerper, im:7149861 antikoerper, zgc:109898 antikoerper, LEDGF antikoerper, p75 antikoerper, PSIP2 antikoerper, DFS70 antikoerper, PAIP antikoerper, p52 antikoerper, AA408851 antikoerper, AU015605 antikoerper, Dfs70 antikoerper, Ledgfa antikoerper, Ledgfb antikoerper, Psip2 antikoerper, Ledgf antikoerper, SBP75 antikoerper, LEDGF/p75 antikoerper, PC4 and SFRS1 interacting protein 1 S homeolog antikoerper, PC4 and SFRS1 interacting protein 1 antikoerper, PC4 and SFRS1 interacting protein 1a antikoerper, LEDGF/p75 transcription factor-like antikoerper, psip1.S antikoerper, PSIP1 antikoerper, psip1a antikoerper, psip1 antikoerper, Psip1 antikoerper

Hintergrund: P52/p75 proteins were first identified through their interaction with transcriptional factors and splicing factors. p75 is encoded by exons 1-15 and contains 530 amino acids, while the alternatively spliced p52 is encoded by exons 1-9 and contains 333 amino acids. Both p52 and p75 have strong interactions with the VP16 activation domain and with various components of the general transcriptional machinery. p52 is the more active transcription activator and has also been shown to recruit the pre-mRNA splicing factor ASF/SF to sites of active transcription. Interestingly, p52/p75 has also been identified as Lens Epithelium-Derived Growth Factor (LEDGF). In serum free media, LEDGF stimulates the growth and survival of a variety of cells, such as lens epithelial cells, retinal photoreceptor cells, COS7 cells, skin fibroblasts, and keratinocytes. In retinal photoreceptor cells, LEDGF is found in the nucleus where it promotes resistance to serum starvation and thermal stress. Thus, the LEDGF gene encodes splice variants that may regulate gene transcription and mRNA splicing of a variety of proteins that are essential for cell growth and survival.
Synonyms: Lens Epithelium-Derived Growth Factor

Molekulargewicht: 75 kDa and/or 52 kDa

Pathways: Chromatin Binding, Ribonucleoprotein Complex Subunit Organization