XPO1 Antikörper (AA 2-122)

Produktnummer ABIN968674

Produktdetails anti-XPO1 Antikörper

Target: XPO1 (Exportin 1 (XPO1))

Bindungsspezifität: AA 2-122

Reaktivität: Human, Maus, Ratte, Hund, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser XPO1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Kreuzreaktivität: Huhn, Hund, Maus, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human hCRM1 aa. 2-122

Klon: 17-Exportin

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968553, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-XPO1 Antikörper (ABIN968674)

Kamura, Hara, Matsumoto, Ishida, Okumura, Hatakeyama, Yoshida, Nakayama, Nakayama: "Cytoplasmic ubiquitin ligase KPC regulates proteolysis of p27(Kip1) at G1 phase." in: Nature cell biology, Vol. 6, Issue 12, pp. 1229-35, (2004) (PubMed).

Ishida, Hara, Kamura, Yoshida, Nakayama, Nakayama: "Phosphorylation of p27Kip1 on serine 10 is required for its binding to CRM1 and nuclear export." in: The Journal of biological chemistry, Vol. 277, Issue 17, pp. 14355-8, (2002) (PubMed).

Massenet, Pellizzoni, Paushkin, Mattaj, Dreyfuss: "The SMN complex is associated with snRNPs throughout their cytoplasmic assembly pathway." in: Molecular and cellular biology, Vol. 22, Issue 18, pp. 6533-41, (2002) (PubMed).

Paraskeva, Izaurralde, Bischoff, Huber, Kutay, Hartmann, Lührmann, Görlich: "CRM1-mediated recycling of snurportin 1 to the cytoplasm." in: The Journal of cell biology, Vol. 145, Issue 2, pp. 255-64, (1999) (PubMed).

Boer, Bonten-Surtel, Grosveld: "Overexpression of the nucleoporin CAN/NUP214 induces growth arrest, nucleocytoplasmic transport defects, and apoptosis." in: Molecular and cellular biology, Vol. 18, Issue 3, pp. 1236-47, (1998) (PubMed).

Fornerod, Ohno, Yoshida, Mattaj: "CRM1 is an export receptor for leucine-rich nuclear export signals." in: Cell, Vol. 90, Issue 6, pp. 1051-60, (1997) (PubMed).

Details zu XPO1

Target: XPO1 (Exportin 1 (XPO1))

Andere Bezeichnung: Exportin-1 (XPO1 Produkte)

Synonyme: CG13387 antikoerper, CRM1 antikoerper, Crm1 antikoerper, DCRM1 antikoerper, Dmel\\CG13387 antikoerper, Emb antikoerper, XPO-1 antikoerper, XPO1 antikoerper, Xpo1 antikoerper, crm1 antikoerper, dCRM1 antikoerper, l(2)k16715 antikoerper, 18.m06600 antikoerper, DDBDRAFT_0183812 antikoerper, DDBDRAFT_0234066 antikoerper, DDB_0183812 antikoerper, DDB_0234066 antikoerper, exportin-1 antikoerper, LOC100220104 antikoerper, emb antikoerper, exp1 antikoerper, Xpo antikoerper, AA420417 antikoerper, Exp1 antikoerper, embargoed antikoerper, exportin 1 antikoerper, putative exportin 1 antikoerper, chromosome region maintenance protein 1 antikoerper, exportin-1 antikoerper, exportin 1 (CRM1 homolog, yeast) antikoerper, exportin 1 S homeolog antikoerper, emb antikoerper, cgd3_3060 antikoerper, Tc00.1047053511725.150 antikoerper, Tb11.01.5940 antikoerper, BBOV_II007220 antikoerper, LMJF_32_1100 antikoerper, xpo1 antikoerper, Xpo1 antikoerper, XPO1 antikoerper, LOC100165622 antikoerper, LOC100633509 antikoerper, xpo1.S antikoerper

Hintergrund: The nuclear pore complex (NPC) is a 125 MDa nuclear envelope complex that mediates nucleocytoplasmic shuttling of RNA and protein. NPC is comprised of more than 30 different proteins, including Ran/TC4, importins, and exportins . Exportin-1(hCRM1/XPO1) is the widely expressed human homologue of the yeast CRM1 protein, which functions in the maintenance of chromatin structure and nuclear export. Exportin-1 has an N-terminal CRIME domain that is homologous to yeast CRM1 and importin-beta. Intracellular localization of Exportin-1 shows association with the NPC and overexpression of Exportin-1 in Xenopus oocytes stimulates Rev and U snRNA export from the nucleus. Exportin-1 also binds other nuclear transport related proteins, such as RanGTP, snurportin-1, and the nucleoporins CAN/Nup214 and Nup50. Overexpression of CAN leads to sequestration of Exportin-1 in the nucleoplasm, which inhibits cell growth and induces apoptosis. In addition, Exportin-1 may export specific proteins, such as Rev and PKI, via binding to a leucine-rich nuclear export signal (NES). Thus, Exportin-1 is an important protein and RNA nuclear export receptor, which may be required for normal cell growth and survival. This antibody is routinely tested by western blot analysis.
Synonyms: CRM1

Molekulargewicht: 112 kDa

Pathways: M Phase