LIG3 Antikörper (AA 2-115)

Produktnummer ABIN968692

Produktdetails anti-LIG3 Antikörper

Target: LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))

Bindungsspezifität: AA 2-115

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LIG3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Kreuzreaktivität: Hund, Ratte (Rattus), Maus

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human DNA Ligase III aa. 2-115

Klon: 7-D Ligase III

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-LIG3 Antikörper (ABIN968692)

Oei, Ziegler: "ATP for the DNA ligation step in base excision repair is generated from poly(ADP-ribose)." in: The Journal of biological chemistry, Vol. 275, Issue 30, pp. 23234-9, (2000) (PubMed).

Taylor, Whitehouse, Caldecott: "The DNA ligase III zinc finger stimulates binding to DNA secondary structure and promotes end joining." in: Nucleic acids research, Vol. 28, Issue 18, pp. 3558-63, (2000) (PubMed).

Mackey, Niedergang, Murcia, Leppard, Au, Chen, de Murcia, Tomkinson: "DNA ligase III is recruited to DNA strand breaks by a zinc finger motif homologous to that of poly(ADP-ribose) polymerase. Identification of two functionally distinct DNA binding regions within DNA ligase III." in: The Journal of biological chemistry, Vol. 274, Issue 31, pp. 21679-87, (1999) (PubMed).

Chen, Tomkinson, Ramos, Mackey, Danehower, Walter, Schultz, Besterman, Husain: "Mammalian DNA ligase III: molecular cloning, chromosomal localization, and expression in spermatocytes undergoing meiotic recombination." in: Molecular and cellular biology, Vol. 15, Issue 10, pp. 5412-22, (1995) (PubMed).

Details zu LIG3

Target: LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))

Andere Bezeichnung: DNA Ligase III (LIG3 Produkte)

Synonyme: orf36 antikoerper, DDBDRAFT_0218523 antikoerper, DDBDRAFT_0232266 antikoerper, DDB_0218523 antikoerper, DDB_0232266 antikoerper, LIG3 antikoerper, wu:fb12c02 antikoerper, wu:fd10b03 antikoerper, wu:fm79b09 antikoerper, LIG2 antikoerper, D11Wsu78e antikoerper, An02g06890 antikoerper, AO090003000668 antikoerper, lig3 antikoerper, DNA ligase III antikoerper, BRCT domain-containing protein antikoerper, DNA ligase 3 antikoerper, ligase III, DNA, ATP-dependent antikoerper, ligase III, DNA, ATP-dependent L homeolog antikoerper, orf36 antikoerper, lig3 antikoerper, LIG3 antikoerper, Lig3 antikoerper, Bm1_22840 antikoerper, ANI_1_2644024 antikoerper, AOR_1_1174154 antikoerper, LOC100634887 antikoerper, lig3.L antikoerper

Hintergrund: Cells have evolved DNA repair pathways that are dedicated to the maintenance of DNA integrity. In such pathways, damaged DNA is excised and the resulting gap is filled by DNA polymerase. Human DNA ligases, ligase I, III, and IV, utilize ATP as a co-factor in DNA joining reactions required for base excision and single strand break repair pathways. All DNA ligases contain an RFPR sequence and an active site motif (ASM) on each side of their catalytic domain. The RFPR is required for transfer of an AMP group from the enzyme to the 5'-phosphate terminus of a DNA nick. In addition, DNA ligase III has an N-terminal zinc finger domain (ZFD) that is homoloqous with the zinc fingers found in poly(ADP-ribose) polymerase (PARP). This domain is not required for DNA ligase activity, but enables DNA ligase III to interact with single strand gaps and single strand flaps. During base excision repair (BER), ATP-dependent ligation requires PARP, DNA polymerase beta, and DNA ligase III interaction with XRCC1 within the BER complex. Thus, DNA ligase III may contain unique protein sequences that allow interaction and repair of specific types of DNA damage.

Molekulargewicht: 103 kDa

Pathways: DNA Reparatur