EIF5A Antikörper (AA 58-154)

Produktnummer ABIN968707

Produktdetails anti-EIF5A Antikörper

Target: EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))

Bindungsspezifität: AA 58-154

Reaktivität: Human, Maus, Ratte, Hund, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EIF5A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), BioImaging (BI)

Kreuzreaktivität: Huhn, Hund, Maus, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
6. Triton is a trademark of the Dow Chemical Company.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human eIF-5a aa. 58-154

Klon: 26-eIF

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-EIF5A Antikörper (ABIN968707)

Hofmann, Reichart, Ewald, Müller, Schmitt, Stauber, Lottspeich, Jockusch, Scheer, Hauber, Dabauvalle: "Cofactor requirements for nuclear export of Rev response element (RRE)- and constitutive transport element (CTE)-containing retroviral RNAs. An unexpected role for actin." in: The Journal of cell biology, Vol. 152, Issue 5, pp. 895-910, (2001) (PubMed).

Xu, Chen: "Hypusine is required for a sequence-specific interaction of eukaryotic initiation factor 5A with postsystematic evolution of ligands by exponential enrichment RNA." in: The Journal of biological chemistry, Vol. 276, Issue 4, pp. 2555-61, (2001) (PubMed).

Koettnitz, Wöhl, Kappel, Lottspeich, Hauber, Bevec: "Identification of a new member of the human eIF-5A gene family." in: Gene, Vol. 159, Issue 2, pp. 283-4, (1995) (PubMed).

Details zu EIF5A

Target: EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))

Andere Bezeichnung: eIF-5a (EIF5A Produkte)

Synonyme: EIF-5A antikoerper, EIF5A1 antikoerper, eIF5AI antikoerper, EIF5A antikoerper, zgc:55504 antikoerper, zgc:77429 antikoerper, wu:fb05b06 antikoerper, wu:fc23f04 antikoerper, wu:fc61c10 antikoerper, AA410058 antikoerper, D19Wsu54e antikoerper, Eif4d antikoerper, Eif5a1 antikoerper, eIF-4D antikoerper, eIF-5A antikoerper, eIF-5A-1 antikoerper, eIF-5A1 antikoerper, zgc:77099 antikoerper, wu:fb17e01 antikoerper, wu:fb63h04 antikoerper, wu:fk81e04 antikoerper, DDBDRAFT_0191442 antikoerper, DDBDRAFT_0218663 antikoerper, DDB_0191442 antikoerper, DDB_0218663 antikoerper, 1296 antikoerper, CG3186 antikoerper, Dmel\\CG3186 antikoerper, anon-EST:fe2H10 antikoerper, eIF5A antikoerper, eIF5a antikoerper, l(2)01296 antikoerper, EIF-5A1 antikoerper, NeIF-5A3 antikoerper, eif5a antikoerper, iff-2 antikoerper, eukaryotic translation initiation factor 5A antikoerper, eukaryotic translation initiation factor 5A2 antikoerper, eukaryotic translation initiation factor 5a antikoerper, putative eukaryotic initiation factor 5a antikoerper, CG3186 gene product from transcript CG3186-RB antikoerper, eukaryotic translation initiation factor 5A-1 antikoerper, eukaryotic translation initiation factor 5A L homeolog antikoerper, EIF5A antikoerper, eif5a2 antikoerper, Eif5a antikoerper, eif5a antikoerper, tif5A antikoerper, Tb11.03.0410 antikoerper, LOC5579111 antikoerper, EIF5A1 antikoerper, eIF-5A antikoerper, LOC107820748 antikoerper, eif5a.L antikoerper

Hintergrund: Initiation of eukaryotic translation involves a series of reactions mediated by multiple eukaryotic initiation factors (eIFs). However, one member of this family, eIF-5a, may have multiple protein functions. eIF-5a was originally identified as an initiation factor due to its binding of ribosomes, and its stimulation of methionyl-puromycin. Regulation of initiation may not be the major function of eIF-5a, since it functions as a targeting protein for the HIV protein, Rev, and the T-cell leukemia virus type 1 protein, Rex. In Xenopus oocytes, eIF-5a interacts with the nucleoporins Nup214, Nup153, Nup98, and Nup62, and is required for Rev-NES interaction with exportin1. These interactions may be involved with eIF-5a nuclear export of Rev protein, and eIF-5a requirement for Rev-mediated viral RNA export. eIF-5a is ubiquitously expressed, and is the only known eukaryotic protein that contains the post-translationally formed hypusine residue. Modification of eIF-5a with hypusine is coupled to cell proliferation, and disruption of this residue in yeast eIF-5a is lethal. Thus, eIF-5a may be an important nuclear transport protein with conserved function in many species.

Molekulargewicht: 18 kDa

Pathways: Regulation of Muscle Cell Differentiation