HIP1R Antikörper (AA 560-772)

Produktnummer ABIN968746

Produktdetails anti-HIP1R Antikörper

Target: HIP1R (Huntingtin Interacting Protein 1 Related (HIP1R))

Bindungsspezifität: AA 560-772

Reaktivität: Human, Ratte, Maus, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HIP1R Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Kreuzreaktivität: Human, Hund, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Hip1R aa. 560-772

Klon: 44-Hip1R

Isotyp: IgG1

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-HIP1R Antikörper (ABIN968746)

Itoh, Koshiba, Kigawa, Kikuchi, Yokoyama, Takenawa: "Role of the ENTH domain in phosphatidylinositol-4,5-bisphosphate binding and endocytosis." in: Science (New York, N.Y.), Vol. 291, Issue 5506, pp. 1047-51, (2001) (PubMed).

Engqvist-Goldstein, Kessels, Chopra, Hayden, Drubin: "An actin-binding protein of the Sla2/Huntingtin interacting protein 1 family is a novel component of clathrin-coated pits and vesicles." in: The Journal of cell biology, Vol. 147, Issue 7, pp. 1503-18, (2000) (PubMed).

Seki, Muramatsu, Sugano, Suzuki, Nakagawara, Ohhira, Hayashi, Hori, Saito: "Cloning, expression analysis, and chromosomal localization of HIP1R, an isolog of huntingtin interacting protein (HIP1)." in: Journal of human genetics, Vol. 43, Issue 4, pp. 268-71, (1999) (PubMed).

Details zu HIP1R

Target: HIP1R (Huntingtin Interacting Protein 1 Related (HIP1R))

Andere Bezeichnung: Hip1R (HIP1R Produkte)

Synonyme: HIP12 antikoerper, HIP3 antikoerper, ILWEQ antikoerper, AA410023 antikoerper, mKIAA0655 antikoerper, HIP1R antikoerper, hip1r antikoerper, hip3 antikoerper, hip12 antikoerper, ilweq antikoerper, DKFZp469N2015 antikoerper, hip1 antikoerper, MGC78813 antikoerper, huntingtin interacting protein 1 related antikoerper, huntingtin interacting protein 1 related b antikoerper, huntingtin interacting protein 1 related L homeolog antikoerper, HIP1R antikoerper, Hip1r antikoerper, hip1rb antikoerper, hip1r antikoerper, hip1r.L antikoerper

Hintergrund: Clathrin is the major protein component in the coat formed around pits and vesicles involved in receptor-mediated endocytosis. Clathrin forms a non-covalently bound triskelion structure composed of three heavy chains (192kDa each) and three light chains (23-25kDa each). A variety of proteins facilitate receptor-mediated endocytosis through association with clathrin-coated vesicles. Huntingtin interacting protein 1 (Hip1) is an actin-binding protein that interacts with Huntingtin protein, and has been implicated in vesicular transport defects found in Huntingtin's disease. Hip1 related protein (Hip1R) is another actin binding protein that contains an epsin NH2-terminal homology (ENTH)domain, three coiled-coil regions, a leucine zipper, and a talin-like actin binding domain. Hip1R mRNA is widely expressed, and Hip1R protein is enriched in the cell cortex and perinuclear region. The ENTH domain of Hip1R is required for binding to phosphatidylinositol-4,5-bisphosphate, and this complex is essential for clathrin-mediated endocytosis. In addition, Hip1R colocalizes with clathrin, AP-2 and endocytosed transferrin. Thus, Hip1R may facilitate interactions between clathrin-coated pits and actin during endocytosis.

Molekulargewicht: 120 kDa