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MAD2L2 Antikörper (AA 81-180)

MAD2L2 Reaktivität: Human, Hund WB, IF Wirt: Maus Monoclonal 14-MAD2B-Rev7 unconjugated
Produktnummer ABIN968795
  • Target Alle MAD2L2 Antikörper anzeigen
    MAD2L2 (MAD2 Mitotic Arrest Deficient-Like 2 (MAD2L2))
    Bindungsspezifität
    • 15
    • 9
    • 4
    • 2
    • 1
    • 1
    AA 81-180
    Reaktivität
    • 41
    • 13
    • 11
    Human, Hund
    Wirt
    • 41
    • 2
    Maus
    Klonalität
    • 33
    • 10
    Monoklonal
    Konjugat
    • 18
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser MAD2L2 Antikörper ist unkonjugiert
    Applikation
    • 31
    • 14
    • 14
    • 13
    • 8
    • 7
    • 7
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF)
    Kreuzreaktivität
    Hund
    Produktmerkmale
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Aufreinigung
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human MAD2B aa. 81-180
    Klon
    14-MAD2B-Rev7
    Isotyp
    IgG1
    Top Product
    Discover our top product MAD2L2 Primärantikörper
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    Related Products: ABIN968586, ABIN967389

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store undiluted at -20° C.
  • Chen, Fang: "MAD2B is an inhibitor of the anaphase-promoting complex." in: Genes & development, Vol. 15, Issue 14, pp. 1765-70, (2001) (PubMed).

    Murakumo, Roth, Ishii, Rasio, Numata, Croce, Fishel: "A human REV7 homolog that interacts with the polymerase zeta catalytic subunit hREV3 and the spindle assembly checkpoint protein hMAD2." in: The Journal of biological chemistry, Vol. 275, Issue 6, pp. 4391-7, (2000) (PubMed).

  • Target
    MAD2L2 (MAD2 Mitotic Arrest Deficient-Like 2 (MAD2L2))
    Andere Bezeichnung
    MAD2B/Rev7 (MAD2L2 Produkte)
    Synonyme
    mad2b antikoerper, 2310033C13Rik antikoerper, MAD2B antikoerper, REV7 antikoerper, mad2B antikoerper, mad2l2-A antikoerper, xMAD2L2 antikoerper, POLZ2 antikoerper, mad2l2 antikoerper, zgc:110299 antikoerper, mitotic arrest deficient 2 like 2 antikoerper, MAD2 mitotic arrest deficient-like 2 antikoerper, mitotic arrest deficient 2 like 2 S homeolog antikoerper, MAD2 mitotic arrest deficient-like 2 (yeast) antikoerper, si:dkey-23c22.2 antikoerper, mad2l2 antikoerper, MAD2L2 antikoerper, Mad2l2 antikoerper, mad2l2.S antikoerper, si:dkey-23c22.2 antikoerper
    Hintergrund
    Progression of the mammalian cell cycle is regulated by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins. These events are of utmost importance at the checkpoints, or transition points, of the cell cycle. MAD2 (Mitotic Arrest-Deficient) is the human homolog of a yeast and Xenopus protein that is essential for spindle assembly during mitosis. Binding of affinity purified polyclonal antibodies to the MAD2 protein has been reported to prevent mitosis of HeLa cells. Furthermore, MAD2 is localized at the kinetochore of condensed chromosomes during mitosis and cells defective in the mitotic checkpoint have reduced levels of MAD2. MAD2 inhibits anaphase-promoting complex (APC) ubiquitin ligase activity,which is critical for controlling transitions in mitosis. This inhibition occurs through binding and suppression of CDC20 activation of APC. MAD2B, also known as Rev7, is a UV revertible gene that has 25% identity with MAD2 and also acts as an inhibitor of APC. Interestingly, MAD2B/Rev7 can bind MAD2 and interacts with the DNA polymerase zeta subunit Rev3. Thus, MAD2B may interact with mitotic checkpoint proteins and DNA repair proteins to regulate mitotic progression. This antibody is routinely tested by western blot analysis.
    Synonyms: Mitotic Arrest Deficient-2B, Rev7
    Molekulargewicht
    26 kDa
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