ASAH1 Antikörper (AA 88-182)

Produktnummer ABIN968809

Dieses Maus Monoklonal-Antikörper erkennt spezifisch ASAH1 in WB und IF. Er zeigt eine Reaktivität gegenüber Human und Hund und wurde in 3+ Publikationen erwähnt.

Kurzübersicht für ASAH1 Antikörper (AA 88-182) (ABIN968809)

Target: ASAH1 (N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1))

Reaktivität: Human, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ASAH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 23-Acid Ceramidase

Produktdetails anti-ASAH1 Antikörper

Bindungsspezifität: AA 88-182

Kreuzreaktivität: Hund

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Acid Ceramidase aa. 88-182

Isotyp: IgG1

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-ASAH1 Antikörper (ABIN968809)

Ferlinz, Kopal, Bernardo, Linke, Bar, Breiden, Neumann, Lang, Schuchman, Sandhoff: "Human acid ceramidase: processing, glycosylation, and lysosomal targeting." in: The Journal of biological chemistry, Vol. 276, Issue 38, pp. 35352-60, (2001) (PubMed).

Strelow, Bernardo, Adam-Klages, Linke, Sandhoff, Krönke, Adam: "Overexpression of acid ceramidase protects from tumor necrosis factor-induced cell death." in: The Journal of experimental medicine, Vol. 192, Issue 5, pp. 601-12, (2000) (PubMed).

Bernardo, Hurwitz, Zenk, Desnick, Ferlinz, Schuchman, Sandhoff: "Purification, characterization, and biosynthesis of human acid ceramidase." in: The Journal of biological chemistry, Vol. 270, Issue 19, pp. 11098-102, (1995) (PubMed).

Details zu ASAH1

Target: ASAH1 (N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1))

Andere Bezeichnung: Acid Ceramidase

Hintergrund: Ceramide is a sphingolipid that exhibits a wide variety of functions, including monocyte differentiation, apoptosis, neurite outgrowth, and Ca2+ transport. It also serves as the precursor of many sphingolipids and anchors these into the outer leaflet of the plasma membrane via hydrophobic interactions. Acid ceramidase is a lysosomal enzyme that was purified from human urine. It is synthesized as a 55kDa precursor protein, which is then processesed into the mature alpha-subunit (13kDa) and beta-subunit (40kDa). Acid ceramidase catalyzes the hydrolysis of ceramide into free fatty acid and sphingosine. Sphingosine, and its phosphorylated form, sphingosine-1-phosphate (SPP), have been shown to inhibit PKC activity and act as a second messenger in cell proliferation and differentiation. Acid ceramidase is also the cause of a lysosomal storage disorder known as Farber’s disease. This disease is characterized by an accumulation of ceramide in tissues, leading to swelling and pain of the joints and extremities, pulmonary insufficiency, and death at an early age. Thus, acid ceramidase is necessary in the hydrolysis of ceramide and is the cause of Farber’s disease.

Molekulargewicht: 13 kDa