DDB1 Antikörper (AA 739-935)

Produktnummer ABIN968852

Produktdetails anti-DDB1 Antikörper

Target: DDB1 (Damage Specific DNA Binding Protein 1 (DDB1))

Bindungsspezifität: AA 739-935

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser DDB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Kreuzreaktivität: Ratte (Rattus), Maus, Hund

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human DDB1 aa. 739-935

Klon: 8-DDB1

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968535, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-DDB1 Antikörper (ABIN968852)

Rapić-Otrin, McLenigan, Bisi, Gonzalez, Levine: "Sequential binding of UV DNA damage binding factor and degradation of the p48 subunit as early events after UV irradiation." in: Nucleic acids research, Vol. 30, Issue 11, pp. 2588-98, (2002) (PubMed).

Sun, Lu, Chao: "Identification of rat DDB1, a putative DNA repair protein, and functional correlation with its damaged-DNA recognition activity." in: Journal of biomedical science, Vol. 9, Issue 4, pp. 371-80, (2002) (PubMed).

Fujiwara, Masutani, Mizukoshi, Kondo, Hanaoka, Iwai: "Characterization of DNA recognition by the human UV-damaged DNA-binding protein." in: The Journal of biological chemistry, Vol. 274, Issue 28, pp. 20027-33, (1999) (PubMed).

Dualan, Brody, Keeney, Nichols, Admon, Linn: "Chromosomal localization and cDNA cloning of the genes (DDB1 and DDB2) for the p127 and p48 subunits of a human damage-specific DNA binding protein." in: Genomics, Vol. 29, Issue 1, pp. 62-9, (1996) (PubMed).

Details zu DDB1

Target: DDB1 (Damage Specific DNA Binding Protein 1 (DDB1))

Andere Bezeichnung: DDB1 (DDB1 Produkte)

Synonyme: DDBA antikoerper, UV-DDB1 antikoerper, XAP1 antikoerper, XPCE antikoerper, XPE antikoerper, XPE-BF antikoerper, DDB1 antikoerper, xpe antikoerper, ddba antikoerper, xap1 antikoerper, xpce antikoerper, xpe-bf antikoerper, uv-ddb1 antikoerper, ddb1 antikoerper, 127kDa antikoerper, AA408517 antikoerper, p127-Ddb1 antikoerper, damage specific DNA binding protein 1 antikoerper, damage-specific DNA binding protein 1, 127kDa antikoerper, DNA damage-binding protein 1 antikoerper, damage-specific DNA binding protein 1 antikoerper, DDB1 antikoerper, ddb1 antikoerper, LOC100187356 antikoerper, Ddb1 antikoerper, ddb-1 antikoerper

Hintergrund: DNA lesions caused by chemical mutagens or radiation are corrected via nucleotide excision repair (NER). The NER system includes multiple proteins involved in xeroderma pigmentosum (XP) disorders, a pathology that causes hypersensitivity to sunlight and higher incidence of skin cancer. The proteins that cause these disorders are XP proteins and include XPA, XPB, XPC, XPD, XPF, and XPG. There are six repair complexes in the NER system composed of 15-18 proteins that include XPA, XPC, XPF, TFIIH, and hHR23B. In addition to these proteins, UV damage DNA-binding (UV-DDB) protein activity has also been associated with the NER system due to the fact that UV-DDB activity is absent in a subset of XPE Ddb- patients. UV-DDB consists of two subunits, DDB1 and DDB2, which can be injected into XPE cells to restore DNA repair synthesis. UV-DDB activity may be involved in the early stages of NER when it may promote recognition of the damaged DNA through DDB2. In addition, DDB1 can bind the histone acetyltransferase p300, which may be important for chromatin remodeling during the early stages of NER. Thus, UV-DDB activity may be important for recognition of specific types of DNA damage during NER.

Molekulargewicht: 127 kDa

Pathways: DNA Reparatur