BUB1B Antikörper (AA 276-388)

Produktnummer ABIN968854

Produktdetails anti-BUB1B Antikörper

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Bindungsspezifität: AA 276-388

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BUB1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human BUBR1 aa. 276-388

Klon: 9-BUBR1

Isotyp: IgG2a

Anwendungsinformationen

Kommentare:

Related Products: ABIN968535, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-BUB1B Antikörper (ABIN968854)

Wu, Lan, Li, Wu, Weinstein, Sakamoto, Dai: "p55CDC/hCDC20 is associated with BUBR1 and may be a downstream target of the spindle checkpoint kinase." in: Oncogene, Vol. 19, Issue 40, pp. 4557-62, (2000) (PubMed).

Chan, Jablonski, Sudakin, Hittle, Yen: "Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC." in: The Journal of cell biology, Vol. 146, Issue 5, pp. 941-54, (1999) (PubMed).

Mizunuma, Hirata, Miyahara, Tsuchiya, Miyakawa: "Role of calcineurin and Mpk1 in regulating the onset of mitosis in budding yeast." in: Nature, Vol. 392, Issue 6673, pp. 303-6, (1998) (PubMed).

Details zu BUB1B

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Andere Bezeichnung: BUBR1 (BUB1B Produkte)

Hintergrund: Accurate chromosome segregation requires that all pairs of sister chromatids become appropriately atttached to mitotic spindles before the onset of anaphase. Cell cycle checkpoints monitor kinetochore-microtubule interactions, so that cell cycle progression can be delayed until proper chromosome attachments are formed. In yeast, Bub1-3 genes are required for proper mitotic delay in response to unattached kinetochores. In mammals, the homologues to yeast Bub1 and Bub3 form a complex that binds kinetochores and has protein kinase activity. BUBR1 is a bub-related kinase homologous to Bub1 and MAD3. BUBR1 contains a nuclear localization signal and two highly conserved domains CD1 and CD2. CD1 is in the N-terminal region and is invoved in kinetochore localization, while CD2 is in the C-terminal region and is involved in kinase activity. BUBR1 is hyperphosphorylated during spindle checkpoint activation, and interacts with p55CDC and APC during spindle checkpoint function. In colorectal cancers, the BUBR1 gene has structural abnormalities that suggest BUBR1 is a tumor suppressor gene. Thus, BUBR1 may be a critical spindle checkpoint kinase and disruption of its function may be related to tumor formation.

Molekulargewicht: 125 kDa