ATP Synthase alpha (AA 113-220) Antikörper

Produktnummer ABIN968856

Produktdetails

Target: ATP Synthase alpha

Bindungsspezifität: AA 113-220

Reaktivität: Huhn, Hund, Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Applikation: Immunofluorescence (IF), Western Blotting (WB)

Kreuzreaktivität: Hund, Ratte (Rattus), Maus, Huhn

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human ATP Synthase alpha aa. 113-220

Klon: 51-ATP Synthase alpha

Isotyp: IgG2a

Anwendungsinformationen

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen

von Ballmoos, Appoldt, Brunner, Granier, Vasella, Dimroth: "Membrane topography of the coupling ion binding site in Na+-translocating F1F0 ATP synthase." in: The Journal of biological chemistry, Vol. 277, Issue 5, pp. 3504-10, (2002) (PubMed).

Xu, Zanotti, Gaballo, Raho, Papa: "F1 and F0 connections in the bovine mitochondrial ATP synthase: the role of the of alpha subunit N-terminus, oligomycin-sensitivity conferring protein (OCSP) and subunit d." in: European journal of biochemistry / FEBS, Vol. 267, Issue 14, pp. 4445-55, (2000) (PubMed).

Lee, Garboczi, Thomas, Pedersen: "Mitochondrial ATP synthase. cDNA cloning, amino acid sequence, overexpression, and properties of the rat liver alpha subunit." in: The Journal of biological chemistry, Vol. 265, Issue 8, pp. 4664-9, (1990) (PubMed).

Antigendetails

Target: ATP Synthase alpha

Hintergrund: ATP synthase is a large enzyme complex that uses an electrochemical H+ or Na+ gradient to synthesize ATP from ADP and Pi, providing the organism with the ATP needed for energy. The complex consists of two major units, F0 and F1. F0 is embedded in the inner membrane of the mitochondria and, due to its hydrophobic nature, translocates protons across this membrane. F1 is the catalytic portion in the matrix region of the mitochondria and is comprised of alpha, beta, gamma, delta, and epsilon subunits at a 3:3:1:1:1 ratio. The alpha subunit is a ubiquitous protein that is highly conserved among species. It has an adenine specific binding site that binds both ATP and ADP. There are two glycine rich regions in the sequence, the A domain and B domain, that are thought to be part of the nucleotide binding domain. It has been demonstrated that the N-terminus of the alpha subunit is necessary for the correct functional and structural connection of F0 to F1. Thus, the alpha subunit is an essential component of the ATP synthase complex and plays a role in properly orienting the F0 and F1 units.

Molekulargewicht: 55 kDa