SPTBN1 Antikörper (AA 2101-2189)

Produktnummer ABIN968882

Produktdetails anti-SPTBN1 Antikörper

Target: SPTBN1 (Spectrin Beta, Non-Erythrocytic 1 (SPTBN1))

Bindungsspezifität: AA 2101-2189

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SPTBN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Kreuzreaktivität: Hund, Maus, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human beta-Spectrin II aa. 2101-2189

Klon: 42-B

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: For western blot analysis use at 1:1000.

Kommentare:

Related Products: ABIN968537

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-SPTBN1 Antikörper (ABIN968882)

Nicolas, Fournier, Galand, Malbert-Colas, Bournier, Kroviarski, Bourgeois, Camonis, Dhermy, Grandchamp, Lecomte: "Tyrosine phosphorylation regulates alpha II spectrin cleavage by calpain." in: Molecular and cellular biology, Vol. 22, Issue 10, pp. 3527-36, (2002) (PubMed).

Hu, Watanabe, Bennett: "Characterization of human brain cDNA encoding the general isoform of beta-spectrin." in: The Journal of biological chemistry, Vol. 267, Issue 26, pp. 18715-22, (1992) (PubMed).

Moon, McMahon: "Generation of diversity in nonerythroid spectrins. Multiple polypeptides are predicted by sequence analysis of cDNAs encompassing the coding region of human nonerythroid alpha-spectrin." in: The Journal of biological chemistry, Vol. 265, Issue 8, pp. 4427-33, (1990) (PubMed).

Details zu SPTBN1

Target: SPTBN1 (Spectrin Beta, Non-Erythrocytic 1 (SPTBN1))

Andere Bezeichnung: beta-Spectrin II (SPTBN1 Produkte)

Synonyme: 9930031C03Rik antikoerper, AL033301 antikoerper, SPTB2 antikoerper, Spnb-2 antikoerper, Spnb2 antikoerper, elf1 antikoerper, elf3 antikoerper, mKIAA4049 antikoerper, ELF antikoerper, betaSpII antikoerper, Spnb1 antikoerper, spectrin beta, non-erythrocytic 1 antikoerper, spectrin, beta, non-erythrocytic 1 antikoerper, Sptbn1 antikoerper, SPTBN1 antikoerper

Hintergrund: Spectrins are central components of the cytoskeleton that form a scaffold below the plasma membrane. Spectrins contain two subunits, alpha and ß, which intertwine to form heterodimers that can self associate into elongated tetramers. alpha-spectin I and ß-spectrin I form heterodimers in red blood cells, while nonerythroid mammalian cells contain heterodimers of alpha-spectin I and II with ß-spectrin I to V. The structure of spectrins includes a succession of triple-helical repeats alongwith various domains, such as SH3 domain, EF hands, PH domains, and binding domains for ankyrin, actin, band 4.1, and calmodulin. alpha-spectrin II is a widely expressed non-erythroid alpha-spectrin that contains an SH3 domain, a calmodulin binding site, and two cleavage sites for proteases, such as calpains and caspase-3. ß-spectrin II is a widely expressed non-erythroid ß-spectrin that contains a C-terminal region that interacts with alpha-spectrins and a PH domain. alpha-spectrin II and ß-spectrin II, like many other spectrins, can form heterodimers that can self associate into tetramers, as well as interact with Band 4.1, F-actin, and other proteins near the plasma membrane. This scaffold of cytoskeletal and plasma membrane proteins is critical for the maintenance of cell structure. This antibody is routinely tested by the Western blot analysis.

Molekulargewicht: 280 kDa

Pathways: Regulation of Actin Filament Polymerization, Protein targeting to Nucleus