OPA1 Antikörper (AA 708-830)

Produktnummer ABIN968891

Produktdetails anti-OPA1 Antikörper

Target: OPA1 (Optic Atrophy 1 (Autosomal Dominant) (OPA1))

Bindungsspezifität: AA 708-830

Reaktivität: Human, Maus, Ratte, Huhn, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser OPA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Kreuzreaktivität: Hund, Ratte (Rattus), Maus, Huhn

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human OPA1 aa. 708-830

Klon: 18-OPA1

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968586, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-OPA1 Antikörper (ABIN968891)

Misaka, Miyashita, Kubo: "Primary structure of a dynamin-related mouse mitochondrial GTPase and its distribution in brain, subcellular localization, and effect on mitochondrial morphology." in: The Journal of biological chemistry, Vol. 277, Issue 18, pp. 15834-42, (2002) (PubMed).

Alexander, Votruba, Pesch, Thiselton, Mayer, Moore, Rodriguez, Kellner, Leo-Kottler, Auburger, Bhattacharya, Wissinger: "OPA1, encoding a dynamin-related GTPase, is mutated in autosomal dominant optic atrophy linked to chromosome 3q28." in: Nature genetics, Vol. 26, Issue 2, pp. 211-5, (2000) (PubMed).

Delettre, Lenaers, Griffoin, Gigarel, Lorenzo, Belenguer, Pelloquin, Grosgeorge, Turc-Carel, Perret, Astarie-Dequeker, Lasquellec, Arnaud, Ducommun, Kaplan, Hamel: "Nuclear gene OPA1, encoding a mitochondrial dynamin-related protein, is mutated in dominant optic atrophy." in: Nature genetics, Vol. 26, Issue 2, pp. 207-10, (2000) (PubMed).

Details zu OPA1

Target: OPA1 (Optic Atrophy 1 (Autosomal Dominant) (OPA1))

Andere Bezeichnung: OPA1 (OPA1 Produkte)

Synonyme: 1200011N24Rik antikoerper, AI225888 antikoerper, AI847218 antikoerper, lilr3 antikoerper, mKIAA0567 antikoerper, MGM1 antikoerper, NPG antikoerper, NTG antikoerper, largeG antikoerper, fk62d06 antikoerper, wu:fb77a10 antikoerper, wu:fk62d06 antikoerper, zgc:92092 antikoerper, OPA1, mitochondrial dynamin like GTPase antikoerper, optic atrophy 1 (autosomal dominant) antikoerper, Opa1 antikoerper, OPA1 antikoerper, opa1 antikoerper

Hintergrund: Three major GTP-binding protein families include trimeric and low molecular weight G-proteins, as well as a family of large proteins homologous to dynamin. The dynamin family contains proteins with diverse structure and function, but highly homologous N-terminal GTPase domains. A subgroup of the dynamin G-protein-binding family includes the mitochondrial proteins Drp1/Dnm1, Mgm1, and OPA1. The latter protein is mutated in dominant optic atrophy, a disease that involves loss of visual acuity and atrophy of the optic nerve. OPA1 is expressed in heart, brain, liver, and kidney. The sequence of OPA1 includes an N-terminal region that contains a mitochondrial targeting domain and three GTP-binding motifs. The overexpression of OPA1 in Cos-7 cells shows co-localization with cytochrome c in mitochondria, and leads to alterations in mitochondrial morphology from a characteristic tubuluar shape to a vesicular pattern. Thus, OPA1 may have roles in mitochondrial biogenesis that are critical for normal cell function. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 80-100 kDa

Pathways: Tube Formation