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beta Actin Antikörper (N-Term)

Name: beta Actin Antikörper (N-Term)
Brand: antibodies-online
SKU: ABIN968977
Price: 587.53 EUR
Availability: InStock

ACTB Reaktivität: Human WB, ELISA, ICC, FACS Wirt: Maus Monoclonal 8H10D10 unconjugated

Western blot analysis using beta-Actin mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), CHO (4), PC12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysate. (beta Actin Antikörper (N-Term))

Flow cytometric analysis of MCF-7 cells using beta Actin mouse mAb (right) and negative control (left). (beta Actin Antikörper (N-Term))

FACS

Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using beta Actin mouse mAb (red, the secondary Ab is Cy3-Goat anti mouse IgG). (beta Actin Antikörper (N-Term))

3 Abbildungen

(5 Referenzen)

Produktnummer ABIN968977

Datenblatt (PDF)

Target Alle beta Actin (ACTB) Antikörper anzeigen

beta Actin (ACTB) (Actin, beta (ACTB))
Bindungsspezifität
38

16

14

6

6

5

5

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
Reaktivität
174

125

106

55

43

41

37

25

23

22

18

17

12

10

8

7

7

6

6

6

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1
Human
Wirt
113

76

5

1

1
Maus
Passende Sekundärantikörper
Klonalität
111

83
Monoklonal
Konjugat
122

12

12

10

3

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser beta Actin Antikörper ist unkonjugiert
Applikation
162

67

50

46

34

30

25

22

13

13

8

6

6

5

5

2

1

1

1
Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Verwendungszweck

beta-Actin Antibody

Aufreinigung

Ascitic fluid

Immunogen

Synthetic peptide corresponding to amino-terminal residues of human beta-Actin, conjugated to KLH.

Klon

8H10D10

Isotyp

IgG2b

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KO Validated ACTB Reaktivität: Human WB, IF, IHC (p), ICC Wirt: Kaninchen Polyclonal unconjugated

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ACTB Reaktivität: Human, Maus, Huhn, Kaninchen, Schwein, Rind (Kuh), Hamster, Ziege, Fisch WB, ELISA Wirt: Maus Monoclonal 2D1D10 unconjugated

Applikationshinweise

ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/200 - 1/1000

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Ascitic fluid containing 0.03 % sodium azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Zou, Ren, Liu, Fu, Chen, Li, Luo, He, Gao, Zeng, Xiong, Li, Huang, Xu, Zhang: "Inhibin B suppresses anoikis resistance and migration through the transforming growth factor-β signaling pathway in nasopharyngeal carcinoma." in: Cancer science, Vol. 109, Issue 11, pp. 3416-3427, (2018) (PubMed).

Rual, Venkatesan, Hao, Hirozane-Kishikawa, Dricot, Li, Berriz, Gibbons, Dreze, Ayivi-Guedehoussou, Klitgord, Simon, Boxem, Milstein, Rosenberg, Goldberg, Zhang, Wong, Franklin, Li, Albala, Lim et al.: "Towards a proteome-scale map of the human protein-protein interaction network. ..." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, (2005) (PubMed).

Xu, Wratten, Charych, Buyske, Firestein, Brzustowicz: "Increased expression in dorsolateral prefrontal cortex of CAPON in schizophrenia and bipolar disorder." in: PLoS medicine, Vol. 2, Issue 10, pp. e263, (2005) (PubMed).

Pederson, Aebi: "Nuclear actin extends, with no contraction in sight." in: Molecular biology of the cell, Vol. 16, Issue 11, pp. 5055-60, (2005) (PubMed).

Bruneel, Labas, Mailloux, Sharma, Royer, Vinh, Pernet, Vaubourdolle, Baudin: "Proteomics of human umbilical vein endothelial cells applied to etoposide-induced apoptosis." in: Proteomics, Vol. 5, Issue 15, pp. 3876-84, (2005) (PubMed).
Target

beta Actin (ACTB) (Actin, beta (ACTB))

Andere Bezeichnung

beta-Actin (ACTB Produkte)

Hintergrund

Description: Beta-actin (PS1TP5-binding protein 1), also known as ACTB, PS1TP5BP1. Entrez Protein NP_001092. It is one of six different actin proteins. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton.Actins are highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Actin is ubiquitously expressed in all eukaryotic cells. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Aliases: PS1TP5BP1, ACTB

Molekulargewicht

42kDa

Gen-ID

60

HGNC

60

UniProt

P60709

Pathways

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction