EIF4E Antikörper

Produktnummer ABIN969094

Produktdetails anti-EIF4E Antikörper

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EIF4E Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Verwendungszweck: EIF4E Antibody

Aufreinigung: Ascitic fluid

Immunogen: Purified recombinant fragment of human EIF4E expressed in E. Coli.

Klon: 5D11

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Referenzen für anti-EIF4E Antikörper (ABIN969094)

Slepenkov, Korneeva, Rhoads: "Kinetic mechanism for assembly of the m7GpppG.eIF4E.eIF4G complex." in: The Journal of biological chemistry, Vol. 283, Issue 37, pp. 25227-37, (2008) (PubMed).

Holm, Byrnes, Johnson, Abreo, Sehon, Alley, Meschonat, Md, Li: "A prospective trial on initiation factor 4E (eIF4E) overexpression and cancer recurrence in node-negative breast cancer." in: Annals of surgical oncology, Vol. 15, Issue 11, pp. 3207-15, (2008) (PubMed).

Details zu EIF4E

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Andere Bezeichnung: EIF4E (EIF4E Produkte)

Hintergrund:

Description: eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.

Aliases: CBP, EIF4F, EIF4E1, EIF4EL1, MGC111573, EIF4E

Molekulargewicht: 25kDa

Gen-ID: 1977

HGNC: 1977

UniProt: P06730

Pathways: BCR Signaling