Cholesterol Oxidase Antikörper (Biotin)
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- Target Alle Cholesterol Oxidase (choS) Produkte
- Cholesterol Oxidase (choS)
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Reaktivität
- Streptomyces
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser Cholesterol Oxidase Antikörper ist konjugiert mit Biotin
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Applikation
- ELISA, Western Blotting (WB), Immunoprecipitation (IP), Dot Blot (DB)
- Hersteller Produkt- Nr.
- 200-106-213-0100
- Hersteller
- Rockland
- Verwendungszweck
- Cholesterol Oxidase Antibody Biotin Conjugated
- Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Cholesterol Oxidase [Microorganism].
- Produktmerkmale
- Synonyms: goat anti-Cholesterol Oxidase Antibody biotin Conjugation, biotin Conjugated goat anti-Cholesterol Oxidase Antibody, choA antibody, choD antibody, choM antibody, choS antibody, COD antibody
- Aufreinigung
- Anti-Cholesterol Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- Immunogen
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Immunogen: Cholesterol Oxidase [Streptomyces]
Immunogen Type: Native Protein
- Isotyp
- IgG
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- Applikationshinweise
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Application Note: Anti-Cholesterol Oxidase has been tested by ELISA, dot blot, and western blot. This product is assayed against 1.0 μg of Cholesterol Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Konzentration
- 10.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Haltbarkeit
- 12 months
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- Target
- Cholesterol Oxidase (choS)
- Andere Bezeichnung
- choA (choS Produkte)
- Hintergrund
- Background: Anti-Cholesterol Oxidase antibody is a member of the GMC oxidoreductase family. Cholesterol Oxidase acts as a catalyst in the oxidation of the 3-beta-hydroxy group of cholesterol and the isomerization of the double bond of new product. Anti-Cholesterol Oxidase is ideal for investigators interested in Metabolism, Signal Transduction, or Cardiovascular research.
- UniProt
- P12676
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