Choline Oxidase Antikörper
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- Target Alle Choline Oxidase Produkte
- Choline Oxidase
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Reaktivität
- Alcaligenes
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser Choline Oxidase Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- Hersteller Produkt- Nr.
- 100-101-094
- Hersteller
- Rockland
- Verwendungszweck
- Choline Oxidase Antibody
- Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Choline Oxidase [Alcaligenes species].
- Produktmerkmale
- Synonyms: Choline oxidase, goat anti-Choline Oxidase Antibody
- Aufreinigung
- This product was prepared from monospecific antiserum by a delipidation and defibrination.
- Immunogen
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Immunogen: Choline Oxidase [Alcaligenes species]
Immunogen Type: Native Protein
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anti-Choline Oxidase antibody (Biotin)
Reaktivität: Alcaligenes WB, ELISA, IP Wirt: Ziege Polyclonal Biotin
anti-Choline Oxidase antibody (HRP)Reaktivität: Alcaligenes WB, ELISA, IP Wirt: Ziege Polyclonal HRP
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- Applikationshinweise
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Application Note: Goat anti-Choline Oxidase Antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Choline Oxidase [Alcaligenes species] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:32,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Konzentration
- 90 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Haltbarkeit
- 12 months
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- Target
- Choline Oxidase
- Abstract
- Choline Oxidase Produkte
- Hintergrund
- Background: Choline Oxidase catalytic activity is Choline + 2 O2 + H2O = betaine + 2 H2O2. This protein is involved in step 1 of the subpathway that synthesizes betaine from choline.
- Gen-ID
- 116367
- UniProt
- P16101
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