Choline Oxidase Antikörper

Produktnummer ABIN97257

Hersteller Produkt- Nr.: 100-101-094

Dieses Anti-Choline Oxidase-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von Choline Oxidase in WB, ELISA und IP. Geeignet für Alcaligenes.

Kurzübersicht für Choline Oxidase Antikörper (ABIN97257)

Target: Choline Oxidase

Reaktivität: Alcaligenes

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Choline Oxidase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Produktdetails anti-Choline Oxidase Antikörper

Hersteller Produkt- Nr.: 100-101-094

Hersteller: Rockland

Verwendungszweck: Choline Oxidase Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Choline Oxidase [Alcaligenes species].

Produktmerkmale: Synonyms: Choline oxidase, goat anti-Choline Oxidase Antibody

Aufreinigung: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Choline Oxidase [Alcaligenes species]

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise:

Application Note: Goat anti-Choline Oxidase Antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Choline Oxidase [Alcaligenes species] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:32,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 90 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu Choline Oxidase

Target: Choline Oxidase

Hintergrund: Background: Choline Oxidase catalytic activity is Choline + 2 O2 + H2O = betaine + 2 H2O2. This protein is involved in step 1 of the subpathway that synthesizes betaine from choline.

Gen-ID: 116367

UniProt: P16101