Cyclin A Antikörper

Produktnummer ABIN97945

Hersteller Produkt- Nr.: 100-401-151

Produktdetails anti-Cyclin A Antikörper

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cyclin A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Hersteller Produkt- Nr.: 100-401-151

Hersteller: Rockland

Verwendungszweck: Cyclin A Antibody

Kreuzreaktivität (Details): Antiserum will specifically react with a 60 kDa Cyclin A protein from human, rat and mouse tissue.

Produktmerkmale: Synonyms: rabbit anti-Cyclin A antibody, CCN1 antibody, CCNA antibody, CCNA2 antibody, Cyclin A2 antibody

Aufreinigung: Anti-Cyclin A was prepared from monospecific antiserum by delipidation and defibrination.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-Cyclin-A Antibody was produced by repeated immunizations with a recombinant protein corresponding to the human cyclin A.

Immunogen Type: Conjugated Peptide

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:100

Application Note: Anti-Cyclin-A antibody has been tested by western blot and immunohistochemistry and is suitable for the detection by immunoblot, ELISA, IHC, and IP of human, rat and mouse Cyclin A.  Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 80 mg/mL

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-Cyclin A Antikörper (ABIN97945)

Rizzolio, Lucchetti, Caligiuri, Marchesi, Caputo, Klein-Szanto, Bagella, Castronovo, Giordano: "Retinoblastoma tumor-suppressor protein phosphorylation and inactivation depend on direct interaction with Pin1." in: Cell death and differentiation, Vol. 19, Issue 7, pp. 1152-61, (2012) (PubMed).

Details zu Cyclin A

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Andere Bezeichnung: CCNA2 (CCNA2 Produkte)

Hintergrund: Background: Anti-Cyclin A detects Cyclin A. Cyclin A is a member of the cyclin family. Cyclin A binds to S phase Cdk2 and is required for the cell to progress through the S phase. Cyclin A/ Cdk2 is inhibited by the complex p21CIP. Anti-Cyclin A Antibody is ideal for investigators involved in Cell Signaling, cell biology and Signal Transduction research.

Gen-ID: 890

NCBI Accession: NP_001228

UniProt: P20248

Pathways: PI3K-Akt Signalweg, Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA