Cyclin B1 Antikörper

Produktnummer ABIN97948

Hersteller Produkt- Nr.: 100-401-152

Produktdetails anti-Cyclin B1 Antikörper

Target: Cyclin B1 (CCNB1)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cyclin B1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Hersteller Produkt- Nr.: 100-401-152

Hersteller: Rockland

Verwendungszweck: Cyclin B1 Antibody

Kreuzreaktivität (Details): Antiserum will specifically react with a 55-62 kDa Cyclin B1 protein from human, rat and mouse tissue.

Produktmerkmale: Synonyms: rabbit anti-cyclin B1 antibody, G2/mitotic-specific cyclin-B1

Aufreinigung: This product was prepared from monospecific antiserum by delipidation and defibrination.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-Cyclin B1 antibody was produced by repeated immunizations of full length fusion protein corresponding to the human gene.

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:500

Application Note: Anti-Cyclin B1 antibody has been tested by western blot and immunohistochemistry and is suitable for use in ELISA, immunoblotting, immunoprecipitation, immunohistochemistry, and other immunological methods requiring high titer and specificity.   Specific conditions for reactivity and detection of Cyclin B1 should be optimized by the end user.  Expect a band approximately 55-66 kDa in size corresponding to Cyclin B1 by western blotting in the appropriate cell lysate or extract. H23 cells may be used as a positive control.

Western Blot Dilution: 1:500 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 80 mg/mL

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-Cyclin B1 Antikörper (ABIN97948)

Wetzel-Strong, Li, Klein, Nishikimi, Caron: "Epicardial-derived adrenomedullin drives cardiac hyperplasia during embryogenesis." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 243, Issue 2, pp. 243-56, (2014) (PubMed).

Details zu Cyclin B1

Target: Cyclin B1 (CCNB1)

Andere Bezeichnung: CCNB1 (CCNB1 Produkte)

Hintergrund: Background: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product forms a complex with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Gen-ID: 891, 14327896

UniProt: P14635

Pathways: Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase