GLB1 Antikörper

Produktnummer ABIN99522

Hersteller Produkt- Nr.: 100-4136

Produktdetails anti-GLB1 Antikörper

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Reaktivität: E. coli

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Hersteller Produkt- Nr.: 100-4136

Hersteller: Rockland

Verwendungszweck: Beta Galactosidase Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Beta Galactosidase [E.

Produktmerkmale: Synonyms: rabbit anti-BETA GALACTOSIDASE Antibody, beta-galactosidase, rabbit anti-beta-gal antibody

Aufreinigung: Beta-Galactosidase Antibody was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Beta Galactosidase (E.coli)

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:500

Application Note: Anti-Beta-Gal Antibody has been tested by western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:65,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 80 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Referenzen für anti-GLB1 Antikörper (ABIN99522)

Cabrera, Charron, Leib: "Neuronal Subtype Determines Herpes Simplex Virus 1 Latency-Associated-Transcript Promoter Activity during Latency." in: Journal of virology, Vol. 92, Issue 13, (2018) (PubMed).

Volckaert, Yuan, Chao, Bell, Sitaula, Szimmtenings, El Agha, Chanda, Majka, Bellusci, Thannickal, Fässler, De Langhe: "Fgf10-Hippo Epithelial-Mesenchymal Crosstalk Maintains and Recruits Lung Basal Stem Cells." in: Developmental cell, Vol. 43, Issue 1, pp. 48-59.e5, (2017) (PubMed).

Katzenell, Leib: "Herpes simplex virus and interferon signaling induce novel autophagic clusters in sensory neurons." in: Journal of virology, (2016) (PubMed).

Shearer, Fragoso, Clagett-Dame, McCaffery: "Astrocytes as a regulated source of retinoic acid for the brain." in: Glia, (2012) (PubMed).

Perry, He, Sugimura, Grindley, Haug, Ding, Li: "Cooperation between both Wnt/{beta}-catenin and PTEN/PI3K/Akt signaling promotes primitive hematopoietic stem cell self-renewal and expansion." in: Genes & development, Vol. 25, Issue 18, pp. 1928-42, (2011) (PubMed).

Details zu GLB1

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Andere Bezeichnung: Beta Galactosidase (GLB1 Produkte)

Hintergrund: Background: Anti Beta Galactosidase Antibody recognizes the enzyme beta galactosidase, or β-galactosidase, that is a component of assays used frequently in genetics, molecular biology (see X-gal) for a blue white screen, and other life sciences. IPTG induces production of β-galactosidase by binding and inhibiting the lac repressor. Since it is highly expressed and accumulated in lysosomes in senescent cells, it is used as a senescence biomarker both in vivo and in vitro in qualitative and quantitative assays, despite its limitations. Anti-beta Galactosidase Antibody is ideal for investigators involved in enzyme research.

NCBI Accession: NP_414878

UniProt: P00722

Pathways: Glycosaminoglycan Metabolic Process