Glucose Oxidase Antikörper

Produktnummer ABIN99703

Hersteller Produkt- Nr.: 100-1137

Produktdetails anti-Glucose Oxidase Antikörper

Target: Glucose Oxidase (GOD)

Reaktivität: Aspergillus niger

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Glucose Oxidase Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Hersteller Produkt- Nr.: 100-1137

Hersteller: Rockland

Verwendungszweck: Glucose Oxidase Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Glucose Oxidase [Aspergillus niger].

Produktmerkmale: Synonyms: goat anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody

Aufreinigung: Anti-glucose oxidase antibody was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Glucose Oxidase [Aspergillus niger]

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-glucose oxidase antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Glucose Oxidase [Aspergillus niger] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product. Optimal concentration should be determined by the researcher.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 90 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Glucose Oxidase

Target: Glucose Oxidase (GOD)

Andere Bezeichnung: Glucose Oxidase (GOD Produkte)

Hintergrund: Background: Glucose oxidase antibody is specific to glucose oxidase enzyme. Glucose oxidase is an oxido-reductase that catalyses the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. Glucose oxidase is commonly used for the determination of free glucose in body fluids (diagnostics), and in the food industry. It also has many applications in biotechnologies, for example enzyme assays for biochemistry including biosensors in nanotechnologies. Glucose oxidase is often extracted from Aspergillus niger.

UniProt: P13006