Glucose Oxidase Antikörper

Produktnummer ABIN99709

Produktdetails anti-Glucose Oxidase Antikörper

Target: Glucose Oxidase (GOD)

Reaktivität: Aspergillus niger

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Glucose Oxidase Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Kreuzreaktivität (Details): Cross reactivity against Glucose Oxidase from other tissues and species may occur but have not been specifically determined.

Reinheit: Anti-Glucose Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Anti-Glucose Oxidase Antibody was produced by repeated immunizations with Aspergillus niger Glucose Oxidase protein.
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-Glucose Oxidase Antibody is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
ELISA Dilution: 1:35.000 - 1:160.000
Immunohistochemistry Dilution: 1:2.000 - 1:8.000
Western Blot Dilution: 1:3500 - 1:16.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu Glucose Oxidase

Target: Glucose Oxidase (GOD)

Andere Bezeichnung: Glucose Oxidase (GOD Produkte)

Hintergrund: Anti-Glucose Oxidase detects glucose oxidase. Glucose oxidase enzyme (GOx) is an oxido-reductase that catalyses the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. In cells, it aids in breaking the sugar down into its metabolites. Glucose oxidase is widely used for the determination of free glucose in body fluids, in vegetal raw material, and in the food industry. It also has many applications in biotechnologies, typically enzyme assays for biochemistry including biosensors in nanotechnologies. Anti-Glucose Oxidase Antibody is ideal for investigators involved in Cell Signaling and Signal Transduction research. Synonyms: Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody

UniProt: P13006