Glucose Oxidase Antikörper

Produktnummer ABIN99709

Hersteller Produkt- Nr.: 200-4137-0100

Produktdetails anti-Glucose Oxidase Antikörper

Target: Glucose Oxidase (GOD)

Reaktivität: Aspergillus niger

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Glucose Oxidase Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Hersteller Produkt- Nr.: 200-4137-0100

Hersteller: Rockland

Verwendungszweck: Glucose Oxidase Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger].

Produktmerkmale: Synonyms: rabbit anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody

Aufreinigung: Anti-Glucose Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Anti-Glucose Oxidase Antibody was produced by repeated immunizations with Aspergillus niger Glucose Oxidase protein.

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:2,000 - 1:8,000

Application Note: Anti-Glucose Oxidase Antibody has been tested by ELISA and western blot and is suitable for IHC. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:3500 - 1:16,000

ELISA Dilution: 1:35,000 - 1:160,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Glucose Oxidase

Target: Glucose Oxidase (GOD)

Andere Bezeichnung: Glucose Oxidase (GOD Produkte)

Hintergrund: Background: Anti-Glucose Oxidase detects glucose oxidase. Glucose oxidase enzyme (GOx) is an oxido-reductase that catalyzes the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. In cells, it aids in breaking the sugar down into its metabolites. Glucose oxidase is widely used for the determination of free glucose in body fluids, in vegetal raw material, and in the food industry. It also has many applications in biotechnologies, typically enzyme assays for biochemistry including biosensors in nanotechnologies. Anti-Glucose Oxidase Antibody is ideal for investigators involved in Cell Signaling and Signal Transduction research.

UniProt: P13006