GLUD1 Antikörper

Produktnummer ABIN99811

Hersteller Produkt- Nr.: 100-4158

Produktdetails anti-GLUD1 Antikörper

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLUD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Hersteller Produkt- Nr.: 100-4158

Hersteller: Rockland

Verwendungszweck: Glutamate Dehydrogenase Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Glutamate Dehydrogenase [Bovine Liver].

Produktmerkmale: Synonyms: rabbit anti-Glutamate Dehydrogenase Antibody, Glutamate dehydrogenase 1 mitochondrial, GDH 1

Aufreinigung: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full length Glutamate Dehydrogenase protein isolated from Bovine Liver.

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise:

Application Note: This antibody has been tested by western blot.  Specific conditions for reactivity should be optimized by the end user. Bovine glutamate dehydrogenase exists as a homohexamer located within the mitochondrial matrix.  Expect a band approximately 56 kDa in size corresponding to glutamate dehydrogenase monomer subunit by western blotting in the appropriate cell or tissue extract. Anti-Glutamate Dehydrogenase Antibody is suitable for use in ELISA.

Western Blot Dilution: 1:1,000 - 1:3,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:4,000 - 1:16,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 85 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Referenzen für anti-GLUD1 Antikörper (ABIN99811)

Pougovkina, te Brinke, Ofman, van Cruchten, Kulik, Wanders, Houten, de Boer: "Mitochondrial protein acetylation is driven by acetyl-CoA from fatty acid oxidation." in: Human molecular genetics, Vol. 23, Issue 13, pp. 3513-22, (2015) (PubMed).

Frigerio, Karaca, De Roo, Mlynárik, Skytt, Carobbio, Pajęcka, Waagepetersen, Gruetter, Muller, Maechler: "Deletion of Glud1 (Glutamate Dehydrogenase 1) in the Central Nervous System affects glutamate handling without altering synaptic transmission." in: Journal of neurochemistry, (2012) (PubMed).

Anthonio, Brees, Baumgart-Vogt, Hongu, Huybrechts, Van Dijck, Mannaerts, Kanaho, Van Veldhoven, Fransen: "Small G proteins in peroxisome biogenesis: the potential involvement of ADP-ribosylation factor 6." in: BMC cell biology, Vol. 10, pp. 58, (2009) (PubMed).

Huybrechts, Van Veldhoven, Hoffman, Zeevaert, de Vos, Demaerel, Brams, Jaeken, Fransen, Cassiman: "Identification of a novel PEX14 mutation in Zellweger syndrome." in: Journal of medical genetics, Vol. 45, Issue 6, pp. 376-83, (2008) (PubMed).

Korolainen, Goldsteins, Nyman, Alafuzoff, Koistinaho, Pirttilae: "Oxidative modification of proteins in the frontal cortex of Alzheimer's disease brain." in: Neurobiology of aging, Vol. 27, Issue 1, pp. 42-53, (2005) (PubMed).

Carobbio, Ishihara, Fernandez-Pascual, Bartley, Martin-Del-Rio, Maechler: "Insulin secretion profiles are modified by overexpression of glutamate dehydrogenase in pancreatic islets." in: Diabetologia, Vol. 47, Issue 2, pp. 266-76, (2004) (PubMed).

Fransen, Vastiau, Brees, Brys, Mannaerts, Van Veldhoven: "Potential role for Pex19p in assembly of PTS-receptor docking complexes." in: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 12615-24, (2004) (PubMed).

Akutsu, Miyazaki: "Biochemical and immunohistochemical studies on tropomyosin and glutamate dehydrogenase in the chicken liver." in: Zoological science, Vol. 19, Issue 3, pp. 275-86, (2002) (PubMed).

Details zu GLUD1

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Andere Bezeichnung: GLUD1 (GLUD1 Produkte)

Hintergrund: Background: Glutamate is a major excitatory neurotransmitter. One enzyme central to the metabolism of glutamate is glutamate dehydrogenase (GDH1, EC 1.4.1.3), that catalyzes the reversible deamination of L-glutamate to 2-oxoglutarate using NAD+ or NADP+. Mammalian GDH is composed of six identical subunits, and the regulation of GDH is very complex.  It has been a major goal to identify the substrate and regulatory binding sites of GDH. It is only in recent years that the three-dimensional structure of GDH from microorganisms is available. Very recently, crystallization of bovine liver GDH was reported for the first time from the mammalian sources. However, remarkably little is known about the detailed structure of mammalian GDH, especially the brain enzymes.

Gen-ID: 281785, 32880221

UniProt: P00366

Pathways: Positive Regulation of Peptide Hormone Secretion, Warburg Effekt