GLUD1 Antikörper

Produktnummer ABIN99811

Produktdetails anti-GLUD1 Antikörper

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLUD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktmerkmale: Concentration Definition: by Refractometry

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full length Glutamate Dehydrogenase protein isolated from Bovine Liver.
Immunogentype:Native

Anwendungsinformationen

Applikationshinweise: This antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Bovine glutamate dehydrogenase exists as a homohexamer located within the mitochondrial matrix.  Expect a band approximately 56 kDa in size corresponding to glutamate dehydrogenase monomer subunit by western blotting in the appropriate cell or tissue extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Restore with deionized water (or equivalent)

Konzentration: 85 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Referenzen für anti-GLUD1 Antikörper (ABIN99811)

Pougovkina, te Brinke, Ofman, van Cruchten, Kulik, Wanders, Houten, de Boer: "Mitochondrial protein acetylation is driven by acetyl-CoA from fatty acid oxidation." in: Human molecular genetics, Vol. 23, Issue 13, pp. 3513-22, (2015) (PubMed).

Frigerio, Karaca, De Roo, Mlynárik, Skytt, Carobbio, Pajęcka, Waagepetersen, Gruetter, Muller, Maechler: "Deletion of Glud1 (Glutamate Dehydrogenase 1) in the Central Nervous System affects glutamate handling without altering synaptic transmission." in: Journal of neurochemistry, (2012) (PubMed).

Anthonio, Brees, Baumgart-Vogt, Hongu, Huybrechts, Van Dijck, Mannaerts, Kanaho, Van Veldhoven, Fransen: "Small G proteins in peroxisome biogenesis: the potential involvement of ADP-ribosylation factor 6." in: BMC cell biology, Vol. 10, pp. 58, (2009) (PubMed).

Huybrechts, Van Veldhoven, Hoffman, Zeevaert, de Vos, Demaerel, Brams, Jaeken, Fransen, Cassiman: "Identification of a novel PEX14 mutation in Zellweger syndrome." in: Journal of medical genetics, Vol. 45, Issue 6, pp. 376-83, (2008) (PubMed).

Korolainen, Goldsteins, Nyman, Alafuzoff, Koistinaho, Pirttilae: "Oxidative modification of proteins in the frontal cortex of Alzheimer's disease brain." in: Neurobiology of aging, Vol. 27, Issue 1, pp. 42-53, (2005) (PubMed).

Carobbio, Ishihara, Fernandez-Pascual, Bartley, Martin-Del-Rio, Maechler: "Insulin secretion profiles are modified by overexpression of glutamate dehydrogenase in pancreatic islets." in: Diabetologia, Vol. 47, Issue 2, pp. 266-76, (2004) (PubMed).

Fransen, Vastiau, Brees, Brys, Mannaerts, Van Veldhoven: "Potential role for Pex19p in assembly of PTS-receptor docking complexes." in: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 12615-24, (2004) (PubMed).

Akutsu, Miyazaki: "Biochemical and immunohistochemical studies on tropomyosin and glutamate dehydrogenase in the chicken liver." in: Zoological science, Vol. 19, Issue 3, pp. 275-86, (2002) (PubMed).

Details zu GLUD1

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Andere Bezeichnung: Glutamate Dehydrogenase (GLUD1 Produkte)

Synonyme: GDH antikoerper, gdh1 antikoerper, GDH 1 antikoerper, glud1 antikoerper, GLUD1 antikoerper, cb719 antikoerper, wu:fb16e02 antikoerper, wu:fb58f12 antikoerper, wu:fe37f03 antikoerper, wu:fj43f02 antikoerper, zgc:192851 antikoerper, zgc:55630 antikoerper, GDH1 antikoerper, GLUD antikoerper, AI118167 antikoerper, Gdh-X antikoerper, Glud antikoerper, Gludl antikoerper, Ac2-281 antikoerper, Gdh1 antikoerper, Gludeha antikoerper, MRG-2 antikoerper, GLUTAMATE DECARBOXYLASE 1 antikoerper, GLUTAMATE DEHYDROGENASE 1 antikoerper, MRG7.13 antikoerper, MRG7_13 antikoerper, glutamate dehydrogenase 1 antikoerper, C2H1orf130 antikoerper, legdh1 antikoerper, cb622 antikoerper, wu:fc33g09 antikoerper, wu:fc66a10 antikoerper, zgc:77186 antikoerper, glutamate dehydrogenase 1 antikoerper, glutamate dehydrogenase 1 S homeolog antikoerper, glutamate dehydrogenase 1, mitochondrial antikoerper, glutamate dehydrogenase 1b antikoerper, glutamate dehydrogenase antikoerper, non-compact myelin associated protein antikoerper, glutamate dehydrogenase 1a antikoerper, GLUD1 antikoerper, GDH1 antikoerper, glud1 antikoerper, glud1.S antikoerper, LOC693461 antikoerper, glud1b antikoerper, Glud1 antikoerper, HACJB3_RS00320 antikoerper, LOC100587725 antikoerper, NCMAP antikoerper, gdh1 antikoerper, glud1a antikoerper

Hintergrund: Glutamate is a major excitatory neurotransmitter. One enzyme central to the metabolism of glutamate is glutamate dehydrogenase (GDH1; EC 1.4.1.3), that catalyzes the reversible deamination of L-glutamate to 2-oxoglutarate using NAD+ or NADP+. Mammalian GDH is composed of six identical subunits, and the regulation of GDH is very complex.  It has been a major goal to identify the substrate and regulatory binding sites of GDH. It is only in recent years that the three-dimensional structure of GDH from microorganisms is available. Very recently, crystallization of bovine liver GDH was reported for the first time from the mammalian sources. However, remarkably little is known about the detailed structure of mammalian GDH, especially the brain enzymes.
Synonyms: Glutamate dehydrogenase 1, mitochondrial GDH 1 EC=1.4.1.3

Gen-ID: 281785, 32880221

UniProt: P00366

Pathways: Positive Regulation of Peptide Hormone Secretion, Warburg Effekt