Glycogen Synthase 1 Antikörper (pSer641)

Produktnummer ABIN99926

Hersteller Produkt- Nr.: 600-401-415

Produktdetails anti-Glycogen Synthase 1 Antikörper

Target: Glycogen Synthase 1 (GYS1) (Glycogen Synthase 1 (Muscle) (GYS1))

Bindungsspezifität: pSer641

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Glycogen Synthase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Hersteller Produkt- Nr.: 600-401-415

Hersteller: Rockland

Verwendungszweck: Glycogen Synthase 1 phospho S641 Antibody

Kreuzreaktivität (Details): Phospho Glycogen Synthase pS641 antibody is directed against human muscle glycogen synthase.

Produktmerkmale: Synonyms: rabbit anti-muscle glycogen synthase pS641 antibody, rabbit anti-glycogen synthase pS641 antibody, Glycogen antibody, Glycogen synthase 1 (muscle) antibody, Glycogen synthase 1 antibody, Glycogen synthase1 antibody, GYS 1 antibody, GYS-1 antibody, GYS1 antibody, Starch synthase muscle antibody

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Human Muscle Glycogen Synthase phospho peptide corresponding to a S641 region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: This phospho specific polyclonal antibody was tested by immunoblotting, immunohistochemistry, and ELISA.  By ELISA the antibody was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide.   Immunoblotting will detect human and mouse muscle glycogen synthase.  Although not tested, this antibody is likely functional in immunoprecipitation.

Western Blot Dilution: 1:1,000 - 1:10,000

ELISA Dilution: 1:20,000 - 1:60,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Phospho GYS Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Glycogen Synthase 1

Target: Glycogen Synthase 1 (GYS1) (Glycogen Synthase 1 (Muscle) (GYS1))

Andere Bezeichnung: GYS1 (GYS1 Produkte)

Hintergrund: Background: Anti-Glycogen synthase 1 pS641 is validated by IHC, Western Blot and ELISA. Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units.  Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000.  GS is subject to regulation through both allosteric and covalent modification and occurs in two forms:  the phosphorylated inactive form, and the dephosphorylated active form.  GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3b that mainly functions to phosphorylate muscle glycogen synthase.  This antibody is specific for the phosphorylated form of GS at S641.   Phosphorylation of GS at S641 has been associated with Antiphospholipid Antibody Syndrome.

Gen-ID: 2997

UniProt: P13807

Pathways: PI3K-Akt Signalweg, AMPK Signaling, Cellular Glucan Metabolic Process