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FACS Antikörper

Fluoreszenz-aktivierte Zellsortierung (FACS) ist eine spezielle Art der Durchflusszytometrie. Sie sortiert ein heterogenes Gemisch biologischer Zellen in zwei oder mehr Behälter, jeweils eine Zelle nach der anderen, auf der Grundlage der spezifischen Lichtstreuungs- und Fluoreszenzeigenschaften jeder Zelle und der an sie gebundenen Antikörper.

Wichtige Targets

Arginase 1 Antibodies

CASP8 Antibodies

CCR2 Antibodies

CD14 Antibodies

FOXP3 Antibodies

IL17 Antibodies

Lamin B1 Antibodies

Alle Flow Cytometry Antibodies

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RFP Antikörper

RFP Reaktivität: Discosoma WB, ELISA, IF, IP, IHC, FACS, IHC (fro), IHC (p) Wirt: Kaninchen Polyclonal unconjugated

Immunofluorescence validation image for anti-Red Fluorescent Protein (RFP) antibody (ABIN1043867)

Western Blot of Rabbit anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunohistochemistry of Anti-RFP Antibody.

Western Blot of Rabbit Anti-RFP MW hu, ms, rt Antibody.

Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody.

Western Blot of Rabbit anti-RFP Antibody.

ELISA Results of Polyclonal Rabbit Anti-RFP Antibody tested against purified RFP protein.

Western Blot of RFP Antibody Pre-Absorbed.

12 Abbildungen

356 Referenzen

Independent Validation

1 Validierung

Produktnummer ABIN129578

Datenblatt (PDF)

beta Actin Antikörper (AA 1-50)

ACTB Reaktivität: Human, Maus, Ratte, Kaninchen, Schwein, Rind (Kuh) WB, ELISA, IHC (p), FACS, IF (cc), IF (p) Wirt: Kaninchen Polyclonal unconjugated

NLRP3 inflammasome activation was involved in P2Y14R deficiency.

Rat lung lysate 30ug, probed (ABIN724340) at 1:200 overnight in 4 °C.

Image kindly submitted by Piotr Mamczur from Wroclaw University.

Human HeLa cell lysates probed with Rabbit Anti-beta Actin Polyclonal Antibody, Unconjugated .

Image kindly submitted by Piotr Mamczur from Wroclaw University.

Hepatic overexpression of HNF4α in rats decreased cholesterol levels in both plasma and liver.

Expression pattern of hepatic cholesterol-regulatory genes under HNF4α overexpression in rat liver.

HNF4α-upregulated SR-BI depends on PPARγ.

High-temperature cultivation of an rhIL-10 expression strain impairs the maturation of G3-pro-rhIL10.

High-temperature cultivation of an rhIL-10 expression strain impairs ER folding capacity.

High-temperature cultivation of an rhIL-10 expression strain impairs ER folding capacity.

(a) Expression of viral N (Nucleoprotein) and G (Glycoprotein) genes mRNA in zebrafish embryonic fibroblast cell line (ZF4) with time after snakehead fish vesiculovirus (SHVV) infection.

(a) Expression of viral N (Nucleoprotein) and G (Glycoprotein) genes mRNA in zebrafish embryonic fibroblast cell line (ZF4) with time after snakehead fish vesiculovirus (SHVV) infection.

Expression of Mx and interferon (IFN) genes in ZF4 with time after SHVV infection.

Expression of Retinoic acid-inducible gene I (RIG-I), Melanoma differentiation-associated gene 5 (MDA5), LGP2T, and LGP2a genes in ZF4 with time after SHVV infection.

(a) The expression of DrLGP2a, DrLGP2b proteins during the infection with SHVV.

Pro-Gly, but not Pro plus Gly (Pro+Gly), promoted the expression and secretion of IGF-1 in the HepG2 cells.

Knockdown of PepT1 eliminated the increase of IGF-1 expression induced by Pro-Gly in the HepG2 cells.

Pro-Gly activated JAK2/STAT5 signaling pathway in a PepT1-dependent manner.

Pro-Gly activated JAK2/STAT5 signaling pathway in a PepT1-dependent manner.

(a) The expression of DrLGP2a, DrLGP2b proteins during the infection with SHVV.

Inhibition of JAK2/STAT5 signaling pathway blocked the promotive effect of Pro-Gly on IGF-1 expression and secretion in the HepG2 cells.

Acute or chronic injection of Pro-Gly, but not Pro plus Gly (Pro+Gly), stimulated IGF-1 expression and secretion in mice.

Acute or chronic injection of Pro-Gly, but not Pro plus Gly (Pro+Gly), stimulated IGF-1 expression and secretion in mice.

Injection of JAK2/STAT5 inhibitor abolished the promotive effect of Pro-Gly on IGF-1 expression and secretion in mice.

Protein expression of MTA1 by Western Blot analysis.

Effects of stable YAP1 silencing in BGC-823 cells and stable YAP1 overexpressing in GES-1 cells on migration and invasion abilities in vitroThe wound healing assay was used to evaluate the migration properties of BGC-823 GC cells (A, G) and GES-1 cells (B, H).

Effects of JQ1 treatment on cell proliferation, migration and invasion in ccRCC cell lines(A) Cell proliferation was determined by XTT assays (*P < 0.

JQ1 treatment inhibited MYC expression in ccRCC cells(A) The expression level of MYC mRNA was significantly upregulated in ccRCC tissues compared with that in normal kidney tissues according to TCGA data (P < 0.

Protein levels of NF-κB and NLRP3/Caspase-1 pathway in CIA rats.

Clinical significance of BRD4 expression in ccRCC according to TCGA data(A) Overall survival (OS) was significantly shortened in patients with high BRD4 expression (top third) compared with that in patients with medium and low BRD4 expression (P = 0.

The effect of LBP on the expression of GFAP, VEGF, and PEDF in the retinal tissue.

Relative expression of AANAT, HIOMT, MT1, and MT2 in COCs of follicles of different sizes.

Effect of E2 and ER antagonist ICI182780 on melatonin production and the expression of AANAT, HIOMT, MT1, and MT2 mRNA and protein in sheep COCs.

Arginine inhibits HIF-1α to reduce LDHA expression after cerebral I/R injury.

Arginine inhibits HIF-1α to reduce LDHA expression after cerebral I/R injury.

Arginine inhibits HIF-1α to reduce LDHA expression after cerebral I/R injury.

Arginine inhibits HIF-1α to reduce LDHA expression after cerebral I/R injury.

Arginine inhibits HIF-1α to reduce LDHA expression after cerebral I/R injury.

NLRP3 inflammasome activation was involved in P2Y14R deficiency.

Upregulated cAMP suppressed acute gouty arthritis in vivo.

Pyroptosis stress was invalidated once cAMP increased by Forskolin in vitro.

Decreased cAMP exaggerated acute gouty arthritis in P2Y14R-KO rats.

43 Abbildungen

270 Referenzen

Produktnummer ABIN724340

Datenblatt (PDF)

Dendra 2 Antikörper

Reaktivität: Koralle WB, FACS, ICC Wirt: Kaninchen Polyclonal unconjugated

Immunocytochemistry validation image for anti-Dendra 2 antibody (ABIN361314)

Localization of Notch::Dendracrispr (anti-Dendra (ABIN361314), green), Histone2B::RFP expressed under the neur promoter (blue) and Delta (anti-Delta, red) in wild-type SOP daughter cells (D, n=9) and in strat SOP daughter cells expressing pnrGAL4>AP-47dsRNA (E, n=20).

Western blotting analysis of Dendra2/GPI fusion protein using rabbit polyclonal antibody PAb (836) on lysates of HEK293T/17 cells transfected with Dendra2/GPI construct, reducing and non-reducing conditions.

WB: line1 – Plum protein 27 kDa, line2 – E.

Localization of NiDendra (anti-Dendra, green), Histone2B::RFP expressed under the neur promoter (blue) and Delta (anti-Delta, red) in wild-type SOP daughter cells and in strat SOP daughter cells expressing pnr-GAL4>AP-47dsRNA.

Single optical section images showing Dendra2-NTR (green), Tomato (red), Bhmt (blue), and DAPI (gray) expression in regenerating livers of the WT and lvl at R48h with control (top panel) and Mtz treatment (bottom panel).

Flow cytometry surface staining pattern of HEK293T/17 cells transfected with Dendra2/GPI construct stained using anti-Dendra2 purified rabbit polyclonal antibody (concentration in sample 2 μg/mL, GAR APC).

Separation of HEK293T/17 cells transfected with Dendra2/GPI construct stained anti-Dendra2 purified rabbit polyclonal antibody (concentration in sample 2 μg/mL, GAR APC, red-filled) from HEK293T/17 cells transfected with Dendra2/GPI construct unstained by primary polyclonal antibody (GAR APC, black-dashed) in flow cytometry analysis (surface staining) of HEK293T17/Dendra2/GPI cell suspension.

8 Abbildungen

16 Referenzen

Independent Validation

1 Validierung

Produktnummer ABIN361314

Datenblatt (PDF)

E-cadherin Antikörper (AA 401-500)

CDH1 Reaktivität: Human, Maus, Ratte WB, FACS, ELISA, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Wirt: Kaninchen Polyclonal unconjugated

Flow Cytometry validation image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 401-500) antibody (ABIN1387847)

Flow Cytometry validation image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 401-500) antibody (ABIN1387847)

Human MCF-7 lysates probed with Anti-E cadherin Polyclonal Antibody, Unconjugated at 1:5000 90min in 37˚C

Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN1387847) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN1387847) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN1387847) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Mouse splenocytes probed with Rabbit Anti-E cadherin Polyclonal Antibody, PE-Cy5.

MCF-7 cells were stained with E cadherin Polyclonal Antibody, Unconjugated at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody.

Image provided by One World Lab validation program.

Image provided by Independent Validation badge 29768.

Image provided by One World Lab validation program.

E-cadherin, N-cadherin, and vimentin expression in PGCCs with budding and control MCF-7 and MDA-MB-231 cells.

Expression of E-cadherin, N-cadherin, and vimentin in human breast tumor tissues.

Expression of E-cadherin, N-cadherin, and vimentin in single stroma PGCCs of human breast cancer.

14 Abbildungen

14 Referenzen

Independent Validation

1 Validierung

Produktnummer ABIN1387847

Datenblatt (PDF)

Caspase 8 Antikörper (AA 411-482)

CASP8 Reaktivität: Human, Maus, Ratte, Affe, Schwein, Schaf WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Western Blotting validation image for anti-Caspase 8 (CASP8) (AA 411-482) antibody (ABIN724205)

Image provided by the Independent Validation Program (badge number 29759).

Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Caspase-8 Polyclonal Antibody, Unconjugated (ABIN724205) 1:200 followed by conjugation to the secondary antibody and DAB staining

L1 mouse liver lysates L2 mouse spleen lysates probed with Anti Caspase 8 Polyclonal Antibody, Unconjugated (ABIN724205) at 1:200 overnight at 4 °C.

Image provided by One World Lab validation program.

Image provided by One World Lab validation program.

6 Abbildungen

15 Referenzen

Independent Validation

1 Validierung

Produktnummer ABIN724205

Datenblatt (PDF)

CD63 Antikörper (AA 120-175)

CD63 Reaktivität: Human, Maus, Ratte, Affe, Rind (Kuh), Hund, Schaf, Kaninchen, Schwein, Pferd, Hamster, Meerschweinchen, Ziege, Esel, Huhn, Cat, Avian FACS, WB, IHC, IF Wirt: Ziege Polyclonal unconjugated

Western Blotting validation image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Western Blotting validation image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Western Blotting validation image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Immunohistochemistry (IHC) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Anti-CD63 Ab at 1/1,000 dilution; Iysate at 50 µg per lane; Rabbit polyclonal to goat lgG (HRP) at 1/10,000 dilution

Immunofluorescence — anti-CD63 Ab in Hepa1-6 cells at 1/50 dilution; cells were fixed with 4% of PFA;

Immuno-localization of CD63 in the hen reproductive trac

Representative immunogold labeling (using TEM) images of CSF-derived EVs from control and prenatally cocaine-exposed male and female monkeys are shown.

Flow cytometry analysis of membrane CD63 level in PMN of control and SCK cows.

Expression of exosomes in bronchoalveolar lavage in Rat BAL fluid Source: PMID36035371

Expression of exosomes in Rat lung tissue Source: PMID36035371

Expression of exosomes in Rat Brain Tissue Source: PMID36035371

Immunofluorescence (IF) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Immunohistochemistry (IHC) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Immunohistochemistry (IHC) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Western Blotting (WB) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Western Blotting (WB) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Immunohistochemistry (IHC) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

18 Abbildungen

10 Referenzen

Independent Validation

3 validations

Produktnummer ABIN1440014

Datenblatt (PDF)

Iba1 Antikörper (C-Term)

IBA1 Reaktivität: Human WB, IHC, ICC, IF, IHC (p), FACS, IHC (fro), IHC (ff) Wirt: Kaninchen Polyclonal unconjugated

Immunofluorescence validation image for anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (C-Term) antibody (ABIN2857032)

IHC-P Image Iba1 antibody detects Iba1 protein on mouse fore brain by immunohistochemical analysis.

IHC-P Image Iba1 antibody detects Iba1 protein on rat hind brain by immunohistochemical analysis.

WB Image Iba1 antibody detects Iba1 protein by western blot analysis.

IHC-P Image Iba1 detects Iba1 protein at cytosol on mouse brain tissue by immunohistochemical analysis.

IHC-P Image Iba1 antibody detects Iba1 protein at cytoplasm on human colon carcinoma stroma by immunohistochemical analysis.

WB Image Sample (30 ug of whole cell lysate) A: Molt-4 , 15% SDS PAGE AIF1 antibody antibody diluted at 1:1000

IHC-P Image Immunohistochemical analysis of paraffin-embedded human hepatoma, using Iba1, antibody at 1:500 dilution.

WB Image Iba1 antibody detects Iba1 protein by Western blot analysis.

ASI administration deactivated microglial activation and reduced inflammatory responses in spinal cords of EAE mice.

Neuropathology in the central nervous system of Fuca1-deficient mice.

W20 attenuated neuropathology and gliosis in the brains of A53T α-synuclein mice.

W20 reduced neuropathology and neuroinflammation in BACHD mice.

Absence of neuroinflammation in RNase H2ΔGFAP mice.

Effects of NADPH oxidase subunit deletion on plasma TNFα levels and microglial activation after LPS injection.

DPI post-treatment attenuated microglia activation in brain after LPS injection.

Expression of microglial marker protein characteristic for the homeostatic or disease-associated stateAImmunoblot analysis of ApoE, CLEC7A, TREM2, and CD68 in lysates of acutely isolated microglia from Grn-/- and Grn+/+ mice (9 months of age, n = 3 per genotype, female).

Development of myeloma with associated spinal glial cell activation.

18 Abbildungen

13 Referenzen

Independent Validation

1 Validierung

Produktnummer ABIN2857032

Datenblatt (PDF)

SOX2 Antikörper

KO Validated SOX2 Reaktivität: Human WB, IF, FACS, ICC, IHC (p), IP, IHC (fro), CUT&RUN Wirt: Kaninchen Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) antibody (ABIN2855074)

Cleavage Under Targets and Release Using Nuclease validation image for anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) antibody (ABIN2855074)

WB Image SOX2 antibody detects SOX2 protein by western blot analysis.

IHC-P Image Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SOX2, antibody at 1:100 dilution.

IP Image Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [N1C3] , or SOX2 antibody [GT1876], Western blot analysis was performed using SOX2 antibody [N1C3], EasyBlot anti-Rabbit IgG was used as a secondary reagent.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using SOX2, antibody at 1:160 dilution.

IHC-P Image SOX2 antibody [N1C3] detects SOX2 protein at nucleus on mouse fore brain by immunohistochemical analysis.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC, using SOX2, antibody at 1:80 dilution.

IHC-P Image SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse esophagus by immunohistochemical analysis.

IHC-P Image SOX2 antibody [N1C3] detects SOX2 protein at nucleus on rat brain stem by immunohistochemical analysis.

FACS Image Flow cytometry on human embryonic stem cells, staining with SOX2 ,antibody at 1:100 dilution(purple) or rabbit IgG (black).

WB Image SOX2 antibody detects SOX2 protein by western blot analysis.

Western blot analysis of CD44, octamer-binding transcription factor 4 (OCT4), sex determining region Y-box 2 (SOX2), SOX9, transketolase (TKT), hypoxia-inducible factor 1 alpha (HIF-α) and phosphoglycerate kinase 1 (PGK-1) in CSC-like and non-CSC UM1 cells (A).

KD of Cops2, but not any other CSN subunits, reduces the expression of Nanog and Oct4.

In vitro characterizations of hc-iPSCs.

S1PR1 activation enhanced NSCs proliferation and neuronal differentiation in hippocampal dentate gyrus (DG) after TBI.

S1PR1-induced NSCs proliferation and neuronal differentiation in TBI rats were affected by MEK/Erk activity.

17 Abbildungen

11 Referenzen

Independent Validation

1 Validierung

Produktnummer ABIN2855074

Datenblatt (PDF)

CD4 Antikörper (AA 385-457)

CD4 Reaktivität: Human, Maus, Ratte, Huhn, Schwein FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Wirt: Kaninchen Polyclonal unconjugated

Immunohistochemistry validation image for anti-CD4 (CD4) (AA 385-457) antibody (ABIN671376)

Immunohistochemistry validation image for anti-CD4 (CD4) (AA 385-457) antibody (ABIN671376)

Immunohistochemistry validation image for anti-CD4 (CD4) (AA 385-457) antibody (ABIN671376)

Immunohistochemistry validation image for anti-CD4 (CD4) (AA 385-457) antibody (ABIN671376)

Mouse spleen cells probed with Rabbit Anti-CD4 Polyclonal Antibody, PE Conjugated for 30 minutes at 1:50.

Formalin-fixed and paraffin embedded human colon carcinoma labeled Anti-CD4 Polyclonal Antibody, Unconjugated (ABIN671376) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded rat lung labeled with Anti-CD4 Polyclonal Antibody, Unconjugated (ABIN671376) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Image was kindly submitted by Dr.

Independently Validated Antibody, image provided by Science Direct, badge number 029634: Mouse spleen stained with anti-CD4 (brown) and counterstained with hematoxylin at 1:400 followed by conjugation to the secondary antibody and DAB staining

Lane 1: CD4 recombinant protein Lane 2: Raji cell lysate probed with at 1:5000 90min in 37˚C.

Paraformaldehyde-fixed, paraffin embedded rat kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.

11 Abbildungen

4 Referenzen

Independent Validation

1 Validierung

Produktnummer ABIN671376

Datenblatt (PDF)

Occludin Antikörper (AA 431-522)

OCLN Reaktivität: Human, Maus, Schwein WB, ELISA, FACS, IF (cc), IF (p), IHC (p) Wirt: Kaninchen Polyclonal unconjugated

Immunofluorescence validation image for anti-Occludin (OCLN) (AA 431-522) antibody (ABIN687337)

Human A549 lysates probed with Rabbit Anti-Occludin Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

HQT regulates epithelial proliferation in the colonic mucosa of mice with DSS-induced acute and chronic colitis.

Formalin-fixed and mouse kidney tissue labeled with Anti-Occludin Polyclonal Antibody, Unconjugated (ABIN687337) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse embryo tissue labeled with Anti-Occludin Polyclonal Antibody, Unconjugated (ABIN687337) at 1:100 followed by conjugation to the secondary antibody and DAB staining

Independently Validated Antibody, image provided by Science Direct, badge number 029632:Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-OCLN/Occludin Polyclonal Antibody, Unconjugated (ABIN687337) at 1:200 followed by conjugation to the secondary antibody and DAPI staining

Formalin-fixed and paraffin embedded mouse embryo tissue labeled with Anti-Occludin Polyclonal Antibody, Unconjugated at 1:100 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and mouse kidney tissue labeled with Anti-Occludin Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Tight junction proteins distribution of IPEC-J2 cells after 24 h incubation of β-conglycinin.

Western blot of tight junction proteins of IPEC-J2 cells after 24 h incubation of β-conglycinin.

10 Abbildungen

5 Referenzen

Independent Validation

1 Validierung

Produktnummer ABIN687337

Datenblatt (PDF)

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