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FACS Antikörper

Fluoreszenz-aktivierte Zellsortierung (FACS) ist eine spezielle Art der Durchflusszytometrie. Sie sortiert ein heterogenes Gemisch biologischer Zellen in zwei oder mehr Behälter, jeweils eine Zelle nach der anderen, auf der Grundlage der spezifischen Lichtstreuungs- und Fluoreszenzeigenschaften jeder Zelle und der an sie gebundenen Antikörper.

Wichtige Targets

Arginase 1 Antibodies

CASP8 Antibodies

CCR2 Antibodies

CD14 Antibodies

FOXP3 Antibodies

IL17 Antibodies

Lamin B1 Antibodies

Alle Flow Cytometry Antibodies

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76.125 Treffer :

Transferrin Receptor Antikörper

TFRC Reaktivität: Human FACS, IP, ICC Wirt: Maus Monoclonal MEM-75 unconjugated

Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD71 (MEM-75) purified antibody (concentration in sample 0.

Surface staining of NALM-6 human peripheral blood pre-B cell leukemia cell line with anti-CD71 (MEM-75) biotin; detection by Streptavidin-PE .

Immunofluorescence staining of CD71 in human HeLa cell line using anti-CD71 (; green).

Immunocytochemistry staining of CD71 in human HeLa cell line using anti-CD71 (MEM-75, green).

Flow cytometry multicolor surface staining pattern of human PHA stimulated lymphocytes using anti-human CD3 (UCHT1) PE antibody (20 μL reagent / 100 μL of peripheral whole blood) and anti-human CD71 (MEM-75) purified antibody (concentration in sample 0.

Separation of human CD3 positive CD71 positive lymphocytes (red-filled) from CD3 negative CD71 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD71 (MEM-75) purified antibody (concentration in sample 0.

6 Abbildungen

1 reference

Produktnummer ABIN94221

Datenblatt (PDF)

Cytokeratin 18 Antikörper

KRT18 Reaktivität: Human WB, FACS, ELISA, IHC (p), IP, ICC Wirt: Maus Monoclonal DC-10 unconjugated

Immunohistochemistry staining of human colon (paraffin sections) using anti-cytokeratin 18 (clone).

Western blotting analysis of human cytokeratin 18 using mouse monoclonal antibody DC-10 on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line, negative control) under non-reducing and reducing conditions.

Detectionof cytokeratin 18 in HeLa cell lysate by mouse monoclonal antibody DC-10 .

Separation of MCF-7 cells (red-filled) from Caco-2 cells (black-dashed) in flow cytometry analysis (intracellular staining) of cells stained using anti-cytokeratin 18 (DC-10) purified antibody (concentration in sample 0,6 μg/mL, GAM APC).

Immunohistochemistry staining of human colon (paraffin sections) using anti-cytokeratin 18 (clone DC-10).

Immunocytochemistry staining of cytokeratin 18 in Hep-2 cells using mouse monoclonal antibody DC-10 ((ABIN94282), diluted 1:400), detected with GAM IgG-Alexa Fluor488 (diluted 1:200, green), cell nuclei stained with PI (1 μg/mL, orange).

6 Abbildungen

4 Referenzen

Produktnummer ABIN94282

Datenblatt (PDF)

CD247 Antikörper (Tyr72)

CD247 Reaktivität: Human, Maus WB, FACS, ICC Wirt: Maus Monoclonal EM-26 unconjugated

Anti-Hu CD3 zeta (pY72) Purified (clone EM-26) specificity verification by WB.

Reactivity of the monoclonal antibodies EM-26 (anti-CD3 zeta phospho-Tyr72) and EM-17 (anti-CD3 zeta phospho-Tyr153) with phosphorylated particular human CD3 zeta mutants.

Detection of phosphorylated CD3 zeta (; light blue) in immunological synapse formed between the lymph node naïve T cells from AND TCR transgenic mice and DCEK cells loaded with MCC peptide, after 20 min.

Immunocytochemistry detection of phosphorylated CD3 zeta (EM-26, light blue) in immunological synapse formed between the lymph node naive T cells from AND TCR transgenic mice and DCEK cells loaded with MCC peptide, after 20 min.

Anti-Hu CD3 zeta (p72) purified antibody (clone EM-26) works in Flow Cytometry application Analysis of the antibody staining was performed on Jurkat cells treated or untreated with pervanadate (PV) prior to the fixation and permeabilization of cell suspension with cold methanol.

Separation of pervanadate stimulated Jurkat cell suspension stained using anti-human CD3 zeta (pY72) (EM-26) purified antibody (concentration in sample 3 μg/mL, GAM APC, red-filled) from pervanadate stimulated Jurkat cell suspension unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

6 Abbildungen

1 reference

Produktnummer ABIN2749060

Datenblatt (PDF)

Integrin beta 2 Antikörper

ITGB2 Reaktivität: Human FACS, WB, IP, IHC (fro), Func, IHC (p) Wirt: Maus Monoclonal MEM-48 unconjugated

Fig.

Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (MEM-48).

Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (Commercially tested by LifeSpan BioSciences.

Western Blotting analysis (non-reducing conditions) of whole cell lysate using anti-human CD18 ().

Separation of human leukocytes (red-filled) from CD18 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD18 (MEM-48) purified antibody (concentration in sample 1 μg/mL, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD18 (MEM-48) purified antibody (concentration in sample 1 μg/mL, GAM APC).

6 Abbildungen

5 Referenzen

Produktnummer ABIN2749019

Datenblatt (PDF)

Cytokeratin 19 Antikörper

KRT19 Reaktivität: Human WB, FACS, ELISA, IHC (p), IP, ICC Wirt: Maus Monoclonal A53-B-A2 unconjugated

Western Blotting analysis (reducing conditions) of whole cell lysate of MCF-7 human breast adenocarcinoma cell line.

Flow cytometry intracellular staining pattern of human peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratin 19 (A53-B/A2) purified antibody (concentration in sample 3 μg/mL, GAM APC).

Immunohistochemistry staining of human prostate (paraffin sections) using anti-cytokeratin 19 (A53-B/A2).

Immunohistochemistry staining (paraffin sections) of cytokeratin 19 in human liver using mouse monoclonal antibody A53-B/A2 (ABIN94290, diluted 1:100), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green), cell nuclei stained with PI (1µg/ml; orange).

Western blotting analysis of human cytokeratin 19 using mouse monoclonal antibody A53-B/A2 on lysates of HT-29 cell line and Jurkat cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions.

Separation of MCF-7 cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratin 19 (A53-B/A2) purified antibody (concentration in sample 3 μg/mL, GAM APC).

6 Abbildungen

2 Referenzen

Produktnummer ABIN94290

Datenblatt (PDF)

CD63 Antikörper

CD63 Reaktivität: Human FACS, IHC (p), IP, ICC Wirt: Maus Monoclonal MEM-259 unconjugated

Flow cytometry analysis of peripheral blood lymphocytes from a patient with allergy to bee venom after stimulation with bee venom, stained with anti-human CD63 FITC.

Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD63 (TEM-259).

Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259 ; green) after co-incubation of living cells with human Transferrin - Dyomics 547 (red); cell nuclei stained with DAPI (blue).

Immunofluorescence staining of CD63 in human HeLa cell line using anti-CD63

Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259 ; green) after co-incubation of living cells with human Transferrin - Dyomics 547; cell nuclei stained with DAPI (blue).

Flow cytometry analysis of IgE-activated peripheral blood stained with anti-human CD63 (MEM-259) purified, GAM-APC.

Immunocytochemistry staining of human skin fibroblasts with anti-CD63 (MEM-259, green) after co-incubation of living cells with human Transferrin - Dyomics 547 (cat.

Immunocytochemistry staining of CD63 in human primary fibroblasts using anti-CD63 (MEM-259, green).

Immunocytochemistry staining of CD63 in human HeLa cell line using anti-CD63 (MEM-259, green).

9 Abbildungen

3 Referenzen

Produktnummer ABIN94214

Datenblatt (PDF)

Cytokeratin 18 Antikörper

KRT18 Reaktivität: Säugetier WB, FACS, ELISA, IHC (p), IP, ICC Wirt: Maus Monoclonal C-04 unconjugated

Immunohistochemistry of paraffin-embedded sections (kidney) Immunohistochemistry staining of human kidney (paraffin-embedded sections) with anti-Cytokeratin 18 (C-04).

Western blotting analysis of human cytokeratin 18 using mouse monoclonal antibody C-04 on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line, negative control) under non-reducing and reducing conditions.

Immunohistochemistry of paraffin-embedded sections (kidney) Immunohistochemistry staining of human kidney (paraffin-embedded sections) Fig.

Intracellular Flow Cytometry analysis Intracellular flow cytometry analysis of cytokeratin expression in HeLa human cervix carcinoma cell line using anti-Cytokeratin 18 () FITC.

Flow cytometry intracellular staining pattern of HeLa cell suspension stained using anti-cytokeratin 18 (C-04) purified antibody (concentration in sample 0,6 μg/ml) GAM FITC.

Immunohistochemistry staining of human kidney (paraffin-embedded sections) with anti-Cytokeratin 18 (C-04).

Separation of HeLa cells stained using anti-cytokeratin 18 (C-04) purified antibody (concentration in sample 0,6 μg/mL, GAM FITC, red-filled) from HeLa cells unstained by primary antibody (GAM FITC, black-dashed) in flow cytometry analysis (intracellular staining) of HeLa cell suspension.

Immunohistochemistry staining of human colon (paraffin-embedded sections) with anti-Cytokeratin 18 (C-04).

8 Abbildungen

4 Referenzen

Produktnummer ABIN94279

Datenblatt (PDF)

ZAP70 Antikörper (C-Term)

ZAP70 Reaktivität: Human WB, FACS, ICC Wirt: Maus Monoclonal ZAP-03 unconjugated

Western Blotting analysis (reducing conditions) of HPB-ALL peripheral blood T cell leukemia cell line (1, 3) and RAMOS human Burkitt lymphoma cell line (2, 4); Lane 1, 2: immunostaining with anti-ZAP-70 ; 0.

Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-ZAP70 (ZAP-03) purified antibody (concentration in sample 9 μg/mL, GAM APC).

Western Blotting analysis (reducing conditions) of HPB-ALL peripheral blood T cell leukemia cell line.

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-ZAP70 (ZAP-03) purified antibody (concentration in sample 9 μg/mL, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue antibody (20 μL reagent / 100 μL of peripheral whole blood).

Separation of human CD3 negative ZAP70 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-ZAP70 (ZAP-03) purified antibody (concentration in sample 9 μg/mL, GAM APC).

Western blotting analysis of human ZAP70 using mouse monoclonal antibody ZAP-03 on lysates of Jurkat cell line and HEK-293T cell line (negative control) under reducing and non-reducing conditions.

6 Abbildungen

1 reference

Produktnummer ABIN125756

Datenblatt (PDF)

Vimentin Antikörper

VIM Reaktivität: Säugetier WB, FACS, IHC (p), ICC Wirt: Maus Monoclonal VI-01 unconjugated

Western Blotting analysis of Vimentin in whole cell lysate of LEP-19 human fibroblast cell line (1,3) and 3T3 mouse fibroblast cell line (2,4).

Immunohistochemistry staining (paraffin sections) of vimentin in human liver using mouse monoclonal antibody VI-01 ((ABIN94491), diluted 1:400), detected with GAM IgM-Alexa Fluor488 (diluted 1:200, green), cell nuclei stained with PI (1 μg/mL, orange).

Immunofluorescence staining (mouse fibroblasts) Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line with anti-Vimentin (VI-01) Dyomics 547.

Immunofluorescence staining (rat basophils)

Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody.

Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody VI-01.

Separation of HeLa cells stained using anti-Vimentin (VI-01) purified antibody (concentration in sample 5 μg/mL, GAM APC, red-filled) from HeLa cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining of methanol permeabilisated cells).

Immunocytochemistry analysis of vimentin in methanol/acetone fixed murine 3T3 cells using mouse monoclonal antibody VI-01.

Anti-Vimentin Purified (VI-01) works in WB application under both reducing and non-reducing conditions.

9 Abbildungen

4 Referenzen

Produktnummer ABIN94491

Datenblatt (PDF)

SIT1 Antikörper

SIT1 Reaktivität: Human WB, IP, FACS Wirt: Maus Monoclonal SIT-01 unconjugated

Western blot of human Jurkat T cell line

Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-SIT (SIT-01) purified antibody (concentration in sample 9 μg/mL, GAM APC).

Separation of human CD3 negative SIT positive lymphocytes (red-filled) from CD3 negative SIT negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-SIT (SIT-01) purified antibody (concentration in sample 9 μg/mL, GAM APC).

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-SIT (SIT-01) purified antibody (concentration in sample 9 μg/mL, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue antibody (20 μL reagent / 100 μL of peripheral whole blood).

Western blotting analysis of human SIT using mouse monoclonal antibody SIT-01 on lysates of Molt-4 and HEK-293T cells under reducing and non-reducing conditions.

Anti-SIT Purified (clone SIT-01) works in WB application.

Anti-SIT Purified (clone SIT-01) specificity verification by WB.

7 Abbildungen

2 Referenzen

Produktnummer ABIN94465

Datenblatt (PDF)

TUBG1 Antikörper (C-Term)

TUBG1 Reaktivität: Human, Ratte, Maus, Rind (Kuh), Huhn, Pflanzen, Protozoa, Schwein WB, ICC, FACS Wirt: Maus Monoclonal TU-30 unconjugated

Western blotting analysis of human gamma-tubulin using mouse monoclonal antibody TU-30 on lysates of various cell lines under reducing and non-reducing conditions.

Separation of MCF-7 cells stained using anti-gamma-Tubulin (TU-30) purified antibody (concentration in sample 9 μg/mL, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

Fig.

Immunofluorescence staining (mouse fibroblasts) Immunofluorescence staining of mouse fibroblasts using anti-gamma-tubulin (; direct conjugate with Dyomics 547, red).

Immunofluorescence staining (mouse embryonal carcinoma cells) Immunofluorescence staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3).

Immunocytochemistry staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3).

Immunocytochemistry staining of murine fibroblasts using anti-gamma-tubulin (TU-30, direct conjugate with Dyomics 547, red).

Immunocytochemistry staining of microtubular networks in 3T3 mouse fibroblasts.

8 Abbildungen

8 Referenzen

Produktnummer ABIN94313

Datenblatt (PDF)

LAT2 Antikörper (Cytoplasmic Domain)

LAT2 Reaktivität: Human, Maus WB, IP, ICC, FACS Wirt: Maus Monoclonal NAP-07 unconjugated

Western blotting analysis of human NTAL using mouse monoclonal antibody NAP-07 on lysates of Raji and Ramos cells and on Jurkat cells (negative control) under reducing and non-reducing conditions.

Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-NTAL (NAP-07) purified antibody (concentration in sample 9 μg/mL, GAM APC).

Confocal microscopy Subcellular localization of NTAL by confocal microscopy in THP-1 human acute monocytic leukemia cell line.

Immunocytochemistry analysis of NTAL by confocal microscopy in THP-1 human acute monocytic leukemia cell line.

Separation of human CD3 negative NTAL positive lymphocytes (red-filled) from CD3 positive NTAL negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-NTAL (NAP-07) purified antibody (concentration in sample 9 μg/mL, GAM APC).

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-NTAL (NAP-07) purified antibody (concentration in sample 9 μg/mL, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue antibody (20 μL reagent / 100 μL of peripheral whole blood).

6 Abbildungen

5 Referenzen

Produktnummer ABIN94439

Datenblatt (PDF)

CD63 Antikörper (Biotin)

CD63 Reaktivität: Human FACS, IHC (p), IP, ICC Wirt: Maus Monoclonal MEM-259 Biotin

Flow cytometry surface staining pattern of IgE stimulated human peripheral whole blood stained using anti-human CD63 (MEM-259) Biotin antibody (concentration in sample 0,6 μg/mL, Streptavidin APC).

Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259 ; green) after co-incubation of living cells with human Transferrin - Dyomics 547 (red); cell nuclei stained with DAPI (blue).

Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259 ; green) after co-incubation of living cells with human Transferrin - Dyomics 547; cell nuclei stained with DAPI (blue).

Immunofluorescence staining of CD63 in human HeLa cell line using anti-CD63

Flow cytometry analysis of peripheral blood lymphocytes from a patient with allergy to bee venom after stimulation with bee venom, stained with anti-human CD63 FITC.

Flow cytometry multicolor surface staining of IgE stimulated peripheral blood mononuclear cells stained using anti-human CD63 (MEM-259) Biotin antibody (concentration in sample 0,6 μg/mL, Streptavidin APC) and anti-human CD203c (NP4D6) PE antibody (20 μL reagent / 100 μL of peripheral whole blood).

Separation of CD63 positive CD203c positive basophil granulocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of IgE stimulated human peripheral whole blood using anti-human CD63 (MEM-259) Biotin antibody (concentration in sample 0,6 μg/mL, Streptavidin APC).

7 Abbildungen

3 Referenzen

Produktnummer ABIN1027697

Datenblatt (PDF)

Growth Hormone Receptor Antikörper (AA 101-200)

GHR Reaktivität: Human, Maus, Ratte, Schwein, Schaf WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Evaluation of the pig GHR transcript with a 4bp insert in vitro.

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-GHR Polyclonal Antibody, Unconjugated (ABIN671481) at 1:400, followed by conjugation to the secondary antibody and DAB staining

Human A549 cell lysates probed with Rabbit Anti-GHR Polyclonal Antibody, Unconjugated .

This image was generously provided by Brandon Menzies, PhD, from The University of Melbourne.

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-GHR Polyclonal Antibody, Unconjugated at 1:400, followed by conjugation to the secondary antibody and DAB staining

Knockout of the pig GHR gene in F2 homozygous pigs (GHR4bp/4bp refers to -/-).

6 Abbildungen

7 Referenzen

Produktnummer ABIN671481

Datenblatt (PDF)

HMGB1 Antikörper (AA 75-170)

HMGB1 Reaktivität: Human, Maus, Ratte WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Lane 1: Mouse Macrophages probed with Rabbit Anti-HMGB1 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded human endometrium carcinoma labeled Anti-HMGB1 Polyclonal Antibody, Unconjugated (ABIN671616) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-HMGB1 Polyclonal Antibody, Unconjugated (ABIN671616) at 1:400, followed by conjugation to the secondary antibody and DAB staining

Human HepG2 cell lysates probed with Rabbit Anti-HMGB1 Polyclonal Antibody, PE-Cy5 conjugated .

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-HMGB1 Polyclonal Antibody, Unconjugated at 1:400, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human endometrium carcinoma labeled Anti-HMGB1 Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

6 Abbildungen

7 Referenzen

Produktnummer ABIN671616

Datenblatt (PDF)

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