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Polyklonale Primärantikörper

Polyklonale Antikörper sind Antikörper, die von verschiedenen B-Zelllinien innerhalb des Wirts sezerniert werden (wohingegen monoklonale Antikörper von einer einzigen Zelllinie stammen). Sie sind eine Sammlung von Immunglobulinmolekülen, die gegen ein spezifisches Antigen reagieren und jeweils ein anderes Epitop identifizieren. Wählen Sie zwischen Eigenschaften wie Wirt, Klon und Antigen, um den von Ihnen benötigten Antikörper zu erhalten.

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Wichtige Targets

beta Actin Antibodies

beta Amyloid Antibodies

Caspase 8 Antibodies

beta Catenin Antibodies

COL1 Antibodies

NFKB1 Antibodies

c-FOS Antibodies

TNF alpha Antibodies

WNT2 Antibodies

Alle Polyklonalen Antibodies

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569.451 Treffer :

GLI3 Antikörper

GLI3 Reaktivität: Human WB, IF, IHC (p), ICC Wirt: Kaninchen Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-GLI Family Zinc Finger 3 (GLI3) antibody (ABIN2855813)

Cleavage Under Targets and Release Using Nuclease validation image for anti-GLI Family Zinc Finger 3 (GLI3) antibody (ABIN2855813)

WB Image GLI3 antibody detects GLI3 protein by Western blot analysis.

WB Image Sample (20 ug of whole cell lysate) A: mouse ESC 5% SDS PAGE antibody diluted at 1:1000

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GLI3, antibody at 1:500 dilution.

IHC-P Image GLI3 antibody detects GLI3 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis.

WB Image Sample (30 ug of whole cell lysate) A: Jurkat 5% SDS PAGE antibody diluted at 1:1000

Expression changes of Hedgehog signaling components.

Western Blot of GLI3 in the SLC mice.

Expression of genes and proteins related to Hh signaling in SLC mice.

10 Abbildungen

Independent Validation

1 Validierung

Produktnummer ABIN2855813

Datenblatt (PDF)

Histone 3 Antikörper (3meLys4)

H3 Reaktivität: Human, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN Wirt: Kaninchen Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (3meLys4) antibody (ABIN6971977)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (3meLys4) antibody (ABIN6971977)

Histone H3K4me3 antibody tested by Western blot.

Histone H3 trimethyl Lys4 antibody tested by dot blot analysis.

Detection of H3K4me3 by immunofluorescence U2OS cells were stained with H3K4me3 antibody at a dilution of 1:500.

Histone H3K4me3 antibody tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with Acute Myelocytic Leukemia (AML) cell line chromatin and 4 μg of Histone H3K4me3 antibody.

6 Abbildungen

Independent Validation

1 Validierung

Produktnummer ABIN6971977

Datenblatt (PDF)

ETV4 Antikörper (AA 1-207)

ETV4 Reaktivität: Human WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Ets Variant 4 (ETV4) (AA 1-207) antibody (ABIN6131217)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Ets Variant 4 (ETV4) (AA 1-207) antibody (ABIN6131217)

Western blot analysis of extracts of various cell lines, using Pea3 / ETV4 Rabbit pAb (ABIN6131217, ABIN6140332, ABIN6140334 and ABIN6221480) at 1:1000 dilution.

Immunofluorescence analysis of U-2 OS cells using Pea3 / ETV4 Rabbit pAb (ABIN6131217, ABIN6140332, ABIN6140334 and ABIN6221480) at dilution of 1:100 (40x lens).

Alignment tracks from CUT&RUN targeting ETV4 in mouse fore limb (11.

Library profiles comparing fragment size distributions on an E-Gel EX 2% agarose gel (Thermo Fisher).

Western blot analysis of extracts of various cell lines, using Pea3 / ETV4 antibody (ABIN6131217, ABIN6140332, ABIN6140334 and ABIN6221480) at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded Human skin using Pea3 / ETV4 antibody (ABIN6131217, ABIN6140332, ABIN6140334 and ABIN6221480) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded Rat skin using Pea3 / ETV4 antibody (ABIN6131217, ABIN6140332, ABIN6140334 and ABIN6221480) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded Mouse skin using Pea3 / ETV4 antibody (ABIN6131217, ABIN6140332, ABIN6140334 and ABIN6221480) at dilution of 1:100 (40x lens).

10 Abbildungen

Independent Validation

1 Validierung

Produktnummer ABIN6140332

Datenblatt (PDF)

CD163 Antikörper (AA 1001-1121)

CD163 Reaktivität: Human, Maus, Ratte, Schwein, Hund WB, FACS, ELISA, IHC (p), IHC (fro), ICC, IF (cc), IF (p) Wirt: Kaninchen Polyclonal unconjugated

Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti CD163 Polyclonal Antibody, Unconjugated (ABIN741570) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Chronic phase glibenclamide reduces inflammation and improves the macrophage phenotype in EAE.

Formalin-fixed and paraffin embedded Human testis tissue labeled with unconjugated Anti-CD163/M130 Polyclonal Antibody, unconjugated at 1:100 for 40 minutes at 37°C followed by labeling Donkey Anti-Rabbit, Cy3 conjugated 1:300, 60 minutes at 37°C.

Mouse splenocytes probed with Rabbit Anti-CD163/M130 Polyclonal Antibody, PE-Cy5.

Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-CD163/M130 Polyclonal Antibody, Unconjugated (ABIN741570) followed by conjugation to the secondary antibody and DAB staining

L1 rat brain, L2 mouse uterus lysates probed (ABIN741570) at 1:200 in 4 °C.

Formalin-fixed and paraffin embedded Human testis tissue labeled with unconjugated Anti-CD163/M130 Polyclonal Antibody, unconjugated at 1:100 for 40 minutes at 37°C followed by labeling Donkey Anti-Rabbit, Cy3 conjugated 1:300, 60 minutes at 37°C.

7 Abbildungen

27 Referenzen

Produktnummer ABIN741570

Datenblatt (PDF)

BAX Antikörper (AA 84-175)

BAX Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh), Kaninchen, Huhn, Zebrafisch (Danio rerio) WB, ELISA, FACS, IP, IHC (p), IF (p), IF (cc), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

L1 rat brain, L2 mouse liver lysates probed at 1:200 in 4˚C.

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-Bax Polyclonal Antibody, Unconjugated (ABIN725390) at 1:800 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-Bax Polyclonal Antibody, unconjugated (ABIN725390) at 1: 800 followed by incubation with conjugated secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rabbit brain tissue labeled with Anti-Bax Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Human HL-60 cells probed with Bax Polyclonal Antibody, Unconjugated (green) at 1:30 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

Formalin-fixed and paraffin embedded rabbit brain tissue labeled with Anti-Bax Polyclonal Antibody, Unconjugated (ABIN725390) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Anti –BAX Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining

L1 rat brain, L2 mouse liver lysates probed (ABIN725390) at 1:200 in 4 °C.

Image provided by One World Lab validation program.

Image provided by One World Lab validation program.

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-Bax Polyclonal Antibody, Unconjugated at 1:800 followed by conjugation to the secondary antibody and DAB staining

11 Abbildungen

34 Referenzen

Produktnummer ABIN725390

Datenblatt (PDF)

NF-kB p65 Antikörper (AA 51-100)

NFkBP65 Reaktivität: Human, Maus, Ratte, Rind (Kuh), Zebrafisch (Danio rerio), Kaninchen, Huhn WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

HCCLM3 lysates probed with Anti-NFKB p65 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin-embedded rat aortic tissue labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody (ABIN668961), Unconjugated at 1:600 followed by conjugation to the secondary antibody and DAB staining

Mouse splenocytes probed with Rabbit Anti-NFkB p65 Polyclonal Antibody, Unconjugated .

Formalin-fixed and paraffin embedded rat spleen labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human endometrium carcinoma labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody , Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Paraformaldehyde-fixed, paraffin embedded rat bladder, Antigen retrieval by boiling in sodium citrate buffer (pH6.

Western blot analysis of P-Akt/Akt ratio, NF-kB and P-NF-kB proteins of rats fed either placebo, (A) B.

7 Abbildungen

30 Referenzen

Produktnummer ABIN668961

Datenblatt (PDF)

CD8 Antikörper (AA 51-150)

CD8 Reaktivität: Human, Maus, Ratte FACS, IHC (fro), WB, ELISA, IHC (p), IF (p), IF (cc) Wirt: Kaninchen Polyclonal unconjugated

Mouse splenocytes probed with Rabbit Anti-CD8 Polyclonal Antibody .

$Quantification and representative immunohistological staining of immune cells in the fracture haematoma/callus.$ IHC (p)

Formalin-fixed and paraffin embedded mouse lymphoma tissue labeled Anti-CD8 Polyclonal Antibody, Unconjugated (ABIN671391) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human oral squamous cell carcinoma labeled Anti-CD8 Polyclonal Antibody, Unconjugated (ABIN671391) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated used at 1:200 dilution for 40 minutes at 37°C.

Image kindly submitted by Amy Beck as part of the free sample program.

Mouse spleen lysate probed with Anti-CD8 Polyclonal Antibody at 1:5000 90min in 37˚C.

H&E and IHC staining for CD8, IL-12 and IFN-γ in subcutaneous LLC1 tumors.

7 Abbildungen

26 Referenzen

Produktnummer ABIN671391

Datenblatt (PDF)

MAP1LC3A Antikörper (cleaved)

MAP1LC3A Reaktivität: Human, Maus WB, IF, IHC (p), ICC Wirt: Kaninchen Polyclonal RB38908 unconjugated

Western blot analysis of anti-cleaved-LC3 (G8a) Pab 1805a in mouse brain tissue lysate.

Immunofluorescent analysis of 4 % paraforldehyde-fixed, 0.

Western blot analysis of lysates from A431 cell line, untreated or treated with chloroquine, 100 ng/mL, using Cleaved-G8a (M1LC3A) 1805a (upper) or Beta-actin (lower).

Immunofluorescent analysis of 4 % paraforldehyde-fixed, 0.

Western blot analysis of lysates from NIH/3T3 cells, untreated or treated with chloroquine, using Cleaved-G8a (M1LC3A) 1805a (upper) or Beta-actin (lower).

Western blot analysis of lysates from NIH/3T3 cells, untreated or treated with chloroquine, using Cleaved-G8a (M1LC3A)(R)(upper) or Beta-actin (lower).

Western blot analysis of lysates from A431 cell line, untreated or treated with chloroquine, 100 ng/mL, using Cleaved-G8a (M1LC3A) Antibody 1805a (upper) or Beta-actin (lower).

7 Abbildungen

35 Referenzen

Produktnummer ABIN388483

Datenblatt (PDF)

beta Actin Antikörper (AA 350-375)

ACTB Reaktivität: Human, Maus, Leopard Frog WB, ELISA, IF, FM Wirt: Kaninchen Polyclonal unconjugated

Western Blot of Rabbit Anti-Beta Actin Antibody.

Immunohistochemistry of Rabbit Anti-Beta Actin Antibody.

Distinct functions of HOXD proteins in regulation of neuronal differentiation.

E2F6 is essential for HOXC9 induction of G1 arrest and repression of cell cycle genes.

Effects of TRPM2 inhibition and RNAi knockdown in cancerous and non-cancerous breast cell lines after chemotherapeutic treatments.

Analysis of poly(ADP-ribose)-mediated caspase-independent cell death in breast adenocarcinoma cells after TRPM2 inhibition and chemotherapeutic treatments.

TRPM2 levels in breast cell lines and knockdown of TRPM2 levels by RNAi silencing.

TRPM2 levels in breast cell lines and knockdown of TRPM2 levels by RNAi silencing.

TRPM2 levels in breast cell lines and knockdown of TRPM2 levels by RNAi silencing.

TRPM2 levels in breast cell lines and knockdown of TRPM2 levels by RNAi silencing.

10 Abbildungen

31 Referenzen

Produktnummer ABIN129657

Datenblatt (PDF)

HIS2AV (pSer137) Antikörper

Reaktivität: Drosophila melanogaster ELISA, IHC, WB Wirt: Kaninchen Polyclonal unconjugated

Western blot using affinity purified anti-histone H2AvD pS137 antibody shows detection of a band at ~15 kDa corresponding to phosphorylated H2AvD (lane 2 arrow-head).

Systemic hormetic responses from muscle-specific DNA damage.

dH1 depletion induces DNA damage.

WB analyses with αdH1, αγH2Av and αH4 of increasing amounts of extracts (lanes 1-3) prepared from siRNAdH1, siRNAlacZ and untreated cells.

WB analysis with αγH2Av and αtubulin at different time points after X-ray irradiation (10Gy) of siRNAdH1 and untreated cells.

DNA damage induced by dH1 depletion associates with R-loops accumulation.

WB analyses with αdH1, αγH2Av and αH4 antibodies of siRNAdH1 and untreated cells sorted at G1-, S and G2/M-phase.

Immunostaining with αγH2Av (green) and αCaspase 3 (red) of wing imaginal discs from dH1-depleted his1RNAi flies upon dATRRNAi co-depletion (bottom) or not (top).

hth mutant nuclei show increased number of DNA breaks.

B, B´) However, hth mutant nuclei show more nuclear signal when stained with the same antibody (see arrows in B´) indicating that they have more breaks in their DNA.

10 Abbildungen

27 Referenzen

Produktnummer ABIN129671

Datenblatt (PDF)

beta Actin Antikörper (AA 2-16)

ACTB Reaktivität: Maus, Ratte, Huhn, Zebrafisch (Danio rerio) WB, IHC, ICC Wirt: Kaninchen Polyclonal unconjugated

Western Blotting validation image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

Western Blotting validation image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

Western Blotting validation image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

Western Blotting (WB) image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

Immunocytochemistry (ICC) image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

Immunohistochemistry (IHC) image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

Indirect immunofluorescence labeling of PFA fixed, paraffin embedded mouse muscle section (dilution 1 : 500; red).

7 Abbildungen

3 Referenzen

Independent Validation

1 Validierung

Produktnummer ABIN1742508

Datenblatt (PDF)

Histone 3 Antikörper (H3K9ac)

H3 Reaktivität: Human, Maus WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Wirt: Kaninchen Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (H3K9ac) antibody (ABIN2667854)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (H3K9ac) antibody (ABIN2667854)

Western blot of Histone H3 acetyl Lys9 antibody (pAb).

ChIP of Histone H3 acetyl Lys9 pAb.

Histone H3 acetyl Lys9 antibody tested by dot blot analysis.

Histone H3 Acetyl Lys9 antibody (pAb) tested by ChIP-Seq.

Immunofluorescence stain of Histone H3 acetyl Lys9 pAb.

7 Abbildungen

3 Referenzen

Independent Validation

1 Validierung

Produktnummer ABIN2668415

Datenblatt (PDF)

Caspase 3 Antikörper (AA 67-175)

CASP3 Reaktivität: Human, Maus, Ratte WB, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

Anti- Caspase3 antibody, Western blotting All lanes: Anti Caspase3 at 0.

Anti-Caspase3 antibody, IHC(P) IHC(P): Mouse Intestine Tissue

Anti-Caspase3 antibody, IHC(P) IHC(P): Rat Intestine Tissue

Anti-Caspase3 antibody, IHC(P) IHC(P): Human Lung Cancer Tissue

Anti- Caspase3 antibody, Western blotting All lanes: Anti Caspase3 at 0.

5 Abbildungen

103 Referenzen

Produktnummer ABIN3043802

Datenblatt (PDF)

SARS-Coronavirus Nucleocapsid Protein (SARS-CoV N) Antikörper

Rockland 200-401-a50 SARS-CoV N Reaktivität: SARS Coronavirus (SARS-CoV), SARS Coronavirus-2 (SARS-CoV-2) ELISA, WB, FACS, FM, IHC Wirt: Kaninchen Polyclonal unconjugated

Western Blot of Rabbit Anti-SARS CoV Nucleocapsid (N) Protein Antibody.

Immunofluorescence of SARS-CoV-2 infection in FFPE cell pellets from in vitro cultured human lung cells infected with SARS-CoV-2.

This immunofluorescence microscopy image shows localization of SARS-CoV-2 infection in human liver ductal organoids.

Immunofluorescence of Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody.

Western blot using anti-SARS CoV Nucleocapsid (N) protein antibody shows detection of a 46-kDa band corresponding to the protein.

Western Blot of Rabbit Anti-SARS-CoV (N) Antibody tested on Caco-2 Cell Lysates before (mock), 24, and 48 hours post infection (hpi).

Immunohistochemistry results showing positive staining in placenta tissue using Anti-SARS-CoV Nucleocapsid (N) Antibody.

Immunohistochemistry of Rabbit Anti-SARS CoV Nucleocapsid Antibody.

ELISA results of Rabbit Anti-SARS-CoV Nucleocapsid (N) Protein.

Immunofluorescence of Rabbit Anti-SARS CoV (N) Protein Antibody.

Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein detection.

Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein synthesis.

Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein detection.

ELISA results of Rabbit Anti-SARS-CoV Nucleocapsid (N) ProteinVariants.

14 Abbildungen

96 Referenzen

Produktnummer ABIN6952544

Datenblatt (PDF)

SNAP Tag Antikörper

ELISA, WB, SEM Wirt: Kaninchen Polyclonal unconjugated

Western Blotting validation image for anti-SNAP Tag antibody (ABIN1573927)

Immunofluorescence validation image for anti-SNAP Tag antibody (ABIN1573927)

Electron Microscopy validation image for anti-SNAP Tag antibody (ABIN1573927)

Lane 1-2: SNAP fusion protein in 293 cell lysate (~ 28 kD) Lane 3: Negative 293 cell lysate Primary antibody: 1 µg/mL Rabbit Anti-SNAP-tag Polyclonal Antibody (ABIN398494) Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (ABIN398323, 1: 10,000) The signal was developed with LumiSensorTM HRP Substrate Kit (ABIN769939)

4 Abbildungen

3 Referenzen

Independent Validation

3 validations

Produktnummer ABIN1573927

Datenblatt (PDF)

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