No cross-reactivity with: Chlamydomonas reinhardtii
Produktmerkmale
Expected / apparent Molecular Weight of the Antigene: 36-40 / 36-40 for Arabidopsis thaliana
Aufreinigung
serum
Immunogen
KLH-conjugated synthetic peptide derived from fully conserved C-terminal consensus motif from plant AOX isoforms 1 and 2 including Arabidopsis thaliana AOX1A (At3g22370) and AOX2 (At3g64210), Solanum lycopersicum Q7XBG9, Oryza sativa Q7XT33,
Applikationshinweise
Recommended Dilution: 1 : 1000 on 10-20 µg of mitochondrial protein/lane with standard ECL detection(WB).
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
For reconstitution add 200 µL of sterile water
Handhabung
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Lagerung
-20 °C
Barreto, Okura, Neshich, Maia, Arruda: "Overexpression of UCP1 in tobacco induces mitochondrial biogenesis and amplifies a broad stress response." in: BMC plant biology, Vol. 14, pp. 144, (2014) (PubMed).
Tsaniklidis, Delis, Nikoloudakis, Katinakis, Aivalakis: "Low temperature storage affects the ascorbic acid metabolism of cherry tomato fruits." in: Plant physiology and biochemistry : PPB / Société française de physiologie végétale, Vol. 84, pp. 149-57, (2014) (PubMed).
Zubo, Potapova, Yamburenko, Tarasenko, Konstantinov, Börner: "Inhibition of the electron transport strongly affects transcription and transcript levels in Arabidopsis mitochondria." in: Mitochondrion, Vol. 19 Pt B, pp. 222-30, (2014) (PubMed).
Wang, Vanlerberghe: "A lack of mitochondrial alternative oxidase compromises capacity to recover from severe drought stress." in: Physiologia plantarum, (2013) (PubMed).
Kornfeld, Heskel, Atkin, Gough, Griffin, Horton, Turnbull: "Respiratory flexibility and efficiency are affected by simulated global change in Arctic plants." in: The New phytologist, Vol. 197, Issue 4, pp. 1161-72, (2013) (PubMed).
Zhang, Peck: "Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana." in: Proteomics, Vol. 11, Issue 9, pp. 1780-8, (2011) (PubMed).
Wang, Li, Liu, He, Bi: "Nitric oxide increases mitochondrial respiration in a cGMP-dependent manner in the callus from Arabidopsis thaliana." in: Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society, (2010) (PubMed).
Target
AOX1/AOX2
(Alternative Oxidase 1/2 (AOX1/AOX2))
Andere Bezeichnung
Plant alternative oxidase 1 and 2 (AOX1/2)
Synonyme
aox2 antikoerper, ALTERNATIVE OXIDASE antikoerper, MSJ1.5 antikoerper, MSJ1_5 antikoerper, alternative oxidase 2 antikoerper, F27G20.12 antikoerper, alternative oxidase 1D antikoerper, Afu2g05060 antikoerper, alternative oxidase 1 antikoerper, ubiquinol oxidase 1, mitochondrial antikoerper, alternative oxidase 2 antikoerper, alternative oxidase 1D antikoerper, alternative oxidase AlxA antikoerper, alternative oxidase antikoerper, aox1 antikoerper, AOX1 antikoerper, AOX2 antikoerper, AOX1D antikoerper, CNA01500 antikoerper, aox antikoerper, AFUA_2G05060 antikoerper, Mmar10_2564 antikoerper, PTOX1 antikoerper, Cseg_3042 antikoerper, VOLCADRAFT_65634 antikoerper
Hintergrund
AGI Code: At3g22370 Alternative oxidases (AOX) are quinol oxidases located in the inner mitochondrial membrane of plants. They function as terminal oxidases in the alternate electron transport pathway, oxidizing ubiquinone to reduce oxygen to water.
Molekulargewicht
expected: 36-40 kDa, apparent: 36-40 for Arabidopsis thaliana