Protein A Resin

Produktnummer ABIN1536564

Produktdetails

Target: Protein A

Reaktivität: Staphylococcus aureus

Applikation: Purification (Purif), Affinity Chromatography (AC)

Verwendungszweck: Protein A Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media.

Produktmerkmale: Protein A Resin can also be used for immunoprecipitation of proteins,protein complexes or antigens. The recombinant protein A ligand is coupled to 4% agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin is greater than 20 mg rabbit IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.

Ligand: Recombinant Streptococcal protein A expressed in E. coli
Number of IgG binding sites per ligand: 5
MW of ligand: Approximately 34 kDa
PI of ligand: 5.17
Degree of substitution: Approximately 2 mg protein A/ml settled resin
Static binding capacity: >20 mg rabbit IgG/ml settled resin
Matrix: spherical agarose, 4%
Average particle size: 90 μm (45-165 μm)

Bead Ligand: Protein A

Bead Matrix: Agarose beads

Bead Size: 90 µm

Anwendungsinformationen

Kommentare:

High temperature heating is not recommended. The agarose melts above 65°C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 1X PBS containing 20% ethanol

Lagerung: 4 °C

Informationen zur Lagerung: Store regenerated Protein A Resin in Binding/Wash Buffer containing 20% ethanol at 2°C to 8°C. Do not freeze.

Haltbarkeit: 18 months

Referenzen

Tu, Xu, Fu, Huang, Wang, Liu, Tao: "Preparation and characterization of novel IgG affinity resin coupling anti-Fc camelid single-domain antibodies." in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, Vol. 983-984, pp. 26-31, (2015) (PubMed).

Jing, Liu, Wang, Zhao: "The steroid hormone 20-hydroxyecdysone via nongenomic pathway activates Ca2+/calmodulin-dependent protein kinase II to regulate gene expression." in: The Journal of biological chemistry, Vol. 290, Issue 13, pp. 8469-81, (2015) (PubMed).

Cheung, Zhu, Lui, Dimitrov, Baron: "Human monoclonal antibody fragments targeting matrilin-3 in growth plate cartilage." in: Pharmaceutical research, Vol. 32, Issue 7, pp. 2439-49, (2015) (PubMed).

Mui, Zhou, Blanchette, Chughtai, Knight, Gruosso, Papadakis, Huang, Park, Gingras, Branton: "The Human Adenovirus Type 5 E4orf4 Protein Targets Two Phosphatase Regulators of the Hippo Signaling Pathway." in: Journal of virology, Vol. 89, Issue 17, pp. 8855-70, (2015) (PubMed).

Ju, Na, Yu, Kim, Jeong, Jung: "Structural consequences of aglycosylated IgG Fc variants evolved for Fc?RI binding." in: Molecular immunology, Vol. 67, Issue 2 Pt B, pp. 350-6, (2015) (PubMed).

Asavapanumas, Ratelade, Verkman: "Unique neuromyelitis optica pathology produced in naïve rats by intracerebral administration of NMO-IgG." in: Acta neuropathologica, Vol. 127, Issue 4, pp. 539-51, (2014) (PubMed).

Wunderley, Brownhill, Stefani, Tabernero, Woodman: "The molecular basis for selective assembly of the UBAP1-containing endosome-specific ESCRT-I complex." in: Journal of cell science, Vol. 127, Issue Pt 3, pp. 663-72, (2014) (PubMed).

Wang, Zhao, Wang: "C-type lectin binds to ?-integrin to promote hemocytic phagocytosis in an invertebrate." in: The Journal of biological chemistry, Vol. 289, Issue 4, pp. 2405-14, (2014) (PubMed).

Mabashi-Asazuma, Kuo, Khoo, Jarvis: "A novel baculovirus vector for the production of nonfucosylated recombinant glycoproteins in insect cells." in: Glycobiology, Vol. 24, Issue 3, pp. 325-40, (2014) (PubMed).

Liu, Cai, Wang, Zhao: "In a nongenomic action, steroid hormone 20-hydroxyecdysone induces phosphorylation of cyclin-dependent kinase 10 to promote gene transcription." in: Endocrinology, Vol. 155, Issue 5, pp. 1738-50, (2014) (PubMed).

Zhang, Verkman: "Longitudinally extensive NMO spinal cord pathology produced by passive transfer of NMO-IgG in mice lacking complement inhibitor CD59." in: Journal of autoimmunity, Vol. 53, pp. 67-77, (2014) (PubMed).

Asavapanumas, Verkman: "Neuromyelitis optica pathology in rats following intraperitoneal injection of NMO-IgG and intracerebral needle injury." in: Acta neuropathologica communications, Vol. 2, pp. 48, (2014) (PubMed).

Payapilly, High: "BAG6 regulates the quality control of a polytopic ERAD substrate." in: Journal of cell science, Vol. 127, Issue Pt 13, pp. 2898-909, (2014) (PubMed).

Gow, Sauter, Pridans, Moffat, Sehgal, Stutchfield, Raza, Beard, Tsai, Bainbridge, Boner, Fici, Garcia-Tapia, Martin, Oliphant, Shelly, Tiwari, Wilson, Smith, Mabbott, Hume: "Characterisation of a novel Fc conjugate of macrophage colony-stimulating factor." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 22, Issue 9, pp. 1580-92, (2014) (PubMed).

Ratelade, Verkman: "Inhibitor(s) of the classical complement pathway in mouse serum limit the utility of mice as experimental models of neuromyelitis optica." in: Molecular immunology, Vol. 62, Issue 1, pp. 104-13, (2014) (PubMed).

Ren, Li, Liu, Cai, Liu, Wang, Zhao: "G-protein ?q participates in the steroid hormone 20-hydroxyecdysone nongenomic signal transduction." in: The Journal of steroid biochemistry and molecular biology, Vol. 144 Pt B, pp. 313-23, (2014) (PubMed).

Lechuga, Baranwal, Li, Naydenov, Kuemmerle, Dugina, Chaponnier, Ivanov: "Loss of ?-cytoplasmic actin triggers myofibroblast transition of human epithelial cells." in: Molecular biology of the cell, Vol. 25, Issue 20, pp. 3133-46, (2014) (PubMed).

Chatterjee, Cooley, Boyd, Mehl, OToole, Sondermann: "Mechanistic insight into the conserved allosteric regulation of periplasmic proteolysis by the signaling molecule cyclic-di-GMP." in: eLife, Vol. 3, pp. e03650, (2014) (PubMed).

Dong, Liu, Cai, Wang, Zhao: "Steroid hormone 20-hydroxyecdysone regulation of the very-high-density lipoprotein (VHDL) receptor phosphorylation for VHDL uptake." in: Insect biochemistry and molecular biology, Vol. 43, Issue 4, pp. 328-35, (2013) (PubMed).

Song, Kim, Kim, Lee, Kim, Park: "IGFBP5 mediates high glucose-induced cardiac fibroblast activation." in: Journal of molecular endocrinology, Vol. 50, Issue 3, pp. 291-303, (2013) (PubMed).

Antigendetails

Target: Protein A

Abstract: Protein A Produkte

Synonyme: alpha protein Bead, repB Bead

Hintergrund: Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding, the Fab region is available for binding antigens.