Cell Lysis Buffer

Produktnummer ABIN5706385

Produktdetails

Applikation: Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Western Blotting (WB)

Aufreinigung: This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

Sterilität: Aseptic filtered

Anwendungsinformationen

Applikationshinweise:

Application Note: The buffer is ready to use for all types of human or animal cells. Lysed cells can be used for Western Blot, co-Immunoprecipitation, Chromatin IP (ChIP), enzyme activity assays, and various other cell based assays.

ChIP Dilution: User Optimized

Western Blot Dilution: User Optimized

Immunoprecipitation Dilution: User Optimized

Kommentare:

Synonyms: Cell Lysis Buffer

Background: Cell Lysis Buffer is used to lyse cells under non-denaturing conditions. If recommended add 1 mM PMSF or other protease inhibitor immediately before use. Keep all reagents and lysates in ice. For adherent cells add 400μL of buffer per 10cm flask once they have been washed in 1X PBS. For suspended cells add 400 μL of buffer per million cells once they have been washed in 1X PBS and pelleted. For tissue lysates add 20μL of buffer per 1 mg of tissue followed by homogenization and sonication.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 X

Buffer:

Buffer: See application note.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 2 weeks