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CCA1 Antikörper

CCA1 Reaktivität: Arabidopsis thaliana WB Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN4966136
  • Target
    CCA1 (Circadian Clock Associated 1 (CCA1))
    Reaktivität
    Arabidopsis thaliana
    Wirt
    Kaninchen
    Klonalität
    Polyklonal
    Applikation
    Western Blotting (WB)
    Kreuzreaktivität (Details)
    Not reactive in: no confirmed exceptions from predicted reactivity are currently known
    Homologie
    please inquire
    Immunogen
    KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CCA1 protein sequence, UniProt:P92973, TAIR:AT2G46830
  • Applikationshinweise
    1 : 500 with most sensitive ECL reagent (WB)
    Kommentare

    Important note about protein extractionTranscription factors are best isolated by freezing nucelar extract in liquid nitrogen after adding the extraction buffer, followed by thawing directly in 100°C heating block not in RT. When the samples were heated for 10 min, spinning of the extract for 5 min is needed to remove the cell debris. From the liquid extract measurement can be done and the rest, frozen in liquid Nitrogen, in aliquots. Frozen samples will not be thawed again to 100°C, but on RT and loaded as soon as they are liquid. Such precautions are necessary to take to be able to detect a transcription factor using antibodies.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    For reconstitution add 50 μL of sterile water.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Target
    CCA1 (Circadian Clock Associated 1 (CCA1))
    Synonyme
    F19D11.11 antikoerper, MYB-RELATED DNA BINDING PROTEIN antikoerper, circadian clock associated 1 antikoerper, circadian clock associated 1 antikoerper, CCA1 antikoerper
    Hintergrund
    CCA1 (Circadian clocl associated 1) encodes a transcriptional repressor that performs overlapping functions with LHY in a regulatory feedback loop that is closely associated with the circadian oscillator of Arabidopsis. Binds to the evening element in the promoter of TOC1 and represses TOC1 transcription. CCA1 and LHY colocalize in the nucleus and form heterodimers in vivo. CCA1 and LHY function synergistically in regulating circadian rhythms of Arabidopsis.
    Molekulargewicht
    67 | 80 kDa
    UniProt
    P92973
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