CLEC4A2 ELISA Kit
CLEC4A2
Reaktivität: Ratte
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951053
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Target
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CLEC4A2
(C-Type Lectin Domain Family 4, Member a2 (CLEC4A2))
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Reaktivität
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Ratte
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Nachweismethode
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Colorimetric
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Methodentyp
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Sandwich ELISA
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Applikation
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ELISA
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Verwendungszweck
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Rat CLEC4A2 ELISA Kit.
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Proben
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytische Methode
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Quantitative
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Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Applikationshinweise
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Optimal working dilution should be determined by the investigator.
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Plattentyp
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Pre-coated
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Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Haltbarkeit
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6 months
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Target
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CLEC4A2
(C-Type Lectin Domain Family 4, Member a2 (CLEC4A2))
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Andere Bezeichnung
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CLEC4A2
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Synonyme
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Clec4a ELISA Kit, Clecsf6 ELISA Kit, DCIR ELISA Kit, Dcir1 ELISA Kit, Dcir2 ELISA Kit, C-type lectin domain family 4, member a2 ELISA Kit, C-type lectin domain family 4, member A2 ELISA Kit, Clec4a2 ELISA Kit
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Gen-ID
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297584
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UniProt
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Q5YIS1
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