CLEC4B2 ELISA Kit
CLEC4B2
Reaktivität: Ratte
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- Target
- CLEC4B2 (C-Type Lectin Domain Family 4, Member b2 (CLEC4B2))
- Reaktivität
- Ratte
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Applikation
- ELISA
- Verwendungszweck
- Rat CLEC4B2 ELISA Kit.
- Proben
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytische Methode
- Quantitative
- Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Plattentyp
- Pre-coated
- Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Haltbarkeit
- 6 months
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- Target
- CLEC4B2 (C-Type Lectin Domain Family 4, Member b2 (CLEC4B2))
- Andere Bezeichnung
- CLEC4B2
- Synonyme
- Aplra1 ELISA Kit, F830043G12Rik ELISA Kit, mDCAR1 ELISA Kit, C-type lectin domain family 4, member B2 ELISA Kit, C-type lectin domain family 4, member b2 ELISA Kit, Clec4b2 ELISA Kit
- Gen-ID
- 450222
- UniProt
- Q67DU9
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