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CYP2A12 Protein (AA 1-492) (Strep Tag)

CYP2A12 Spezies: Maus Wirt: Tobacco (Nicotiana tabacum) Recombinant > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC). ELISA, SDS, WB
Produktnummer ABIN3120512
  • Target
    CYP2A12 (Cytochrome P450, Family 2, Subfamily A, Polypeptide 12 (CYP2A12))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-492
    Spezies
    Maus
    Quelle
    Tobacco (Nicotiana tabacum)
    Aufreinigungstag / Konjugat
    Dieses CYP2A12 Protein ist gelabelt mit Strep Tag.
    Applikation
    ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequenz
    MLGSGLLLLA ILAFLSVMVL VSVWQQKIRG KLPPGPIPLP FIGNYLQLNR KDVYSSITQL QEHYGPVFTI HLGPRRVVVL YGYDAVKEAL VDHAEEFSGR GEQATFNTLF KGYGVAFSNG ERAKQLRRFS IATLRDFGMG KRGVEERIQE EAGCLIKMLQ GTCGAPIDPT IYLSKTASNV ISSIVFGDRF NYEDKEFLSL LQMMGQVNKF AASPTGQLYD MFHSVMKYLP GPQQQIIKDS HKLEDFMIQK VKQNQSTLDP NSPRDFIDSF LIHMQKEKYV NSEFHMKNLV MTSLNLFFAG SETVSSTLRY GFLLLMKHPD VEAKVHEEID RVIGRNRQPQ YEDHMKMPYT QAVINEIQRF SNFAPLGIPR RITKDTSFRG FFLPKGTEVF PILGSLMTDP KFFSSPKDFN PQHFLDDKGQ LKKIPAFLPF STGKRFCLGD SLAKMELFLF FTTILQNFRF KFPRKLEDIN ESPTPEGFTR IIPKYTMSFV PI
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Produktmerkmale
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
    Reinheit
    > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    CYP2A12 (Cytochrome P450, Family 2, Subfamily A, Polypeptide 12 (CYP2A12))
    Andere Bezeichnung
    Cyp2a12
    Synonyme
    cytochrome P450, family 2, subfamily a, polypeptide 12 Protein, Cyp2a12 Protein
    Hintergrund
    Cytochrome P450 2A12 (EC 1.14.14.1) (CYPIIA12) (Steroid hormones 7-alpha-hydroxylase) (Testosterone 7-alpha-hydroxylase),FUNCTION: Highly active in the 7-alpha-hydroxylation of testosterone.
    Molekulargewicht
    56.2 kDa
    UniProt
    P56593
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