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D7Ertd443e Protein (AA 1-644) (Strep Tag)

D7ERTD443E Spezies: Maus Wirt: Tobacco (Nicotiana tabacum) Recombinant > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC). ELISA, SDS, WB
Produktnummer ABIN3131078
  • Target
    D7Ertd443e (D7ERTD443E) (DNA Segment, Chr 7, ERATO Doi 443, Expressed (D7ERTD443E))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-644
    Spezies
    Maus
    Quelle
    Tobacco (Nicotiana tabacum)
    Aufreinigungstag / Konjugat
    Dieses D7Ertd443e Protein ist gelabelt mit Strep Tag.
    Applikation
    ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequenz
    MISPVVISRL IDEKKSMENG AILPQAIAQP QLCPTKPALA RRDGVSMHRR FALSPDRLGI LTPSDDQGLE TEPLSTGDNL GKGSHSGFSS ITITARRVGP PASSLVWDTF RDPLCPKCKA KDALFQEPPV LAGDAHLCQH NRPFTCTESP SNGSVEGMKV FQAHSRLSAR QDYWVTHTND NEDSFSSDNS PSRKVPLVFS SCVHFRVSQQ CPNAIYYLDK SLSVPLERPQ IASPKMHRSV LSLSLRCSSH QLTADGVDSS ANGEPISTAL SQELSEGKQD LLGPQWGQPQ GGHWKESPAL VPVHLGSGTC PRTGSPPLEN VKFADVGRNQ VPVRKEKEDH ATCTSSSHTN QLSIHIPGWS YRAETKVLSG SKKQQQEAQR TLPAFPVGQK TIKHFPPEGD SSPSSDGQPS ILSESNERQH PYFMIPRVPL PGFYCPLQTG CASLQEDGAV QIETHFPKDY TCCDLVVKLK ECEKNEDPTV TPEPSPATPS PSTPEGAQSS DPSEDSYEPL LASSMTLQEA LEVHRPQFIS RSQERLQKLK RMVQQRKTQQ KESLGQKQSL LPVRANKKQF TIPHPLSDNL FKPKERCISE KEMHMRSKRI YNNLPEVKKK KEEQKKRMIL QSNRLRAEVF KKQLLDQLLQ RNAV
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Produktmerkmale
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
    Reinheit
    > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    D7Ertd443e (D7ERTD443E) (DNA Segment, Chr 7, ERATO Doi 443, Expressed (D7ERTD443E))
    Andere Bezeichnung
    D7Ertd443e
    Synonyme
    4933400E14Rik Protein, FATS Protein, Gm497 Protein, DNA segment, Chr 7, ERATO Doi 443, expressed Protein, D7Ertd443e Protein
    Hintergrund
    (E2-independent) E3 ubiquitin-conjugating enzyme FATS (EC 2.3.2.-) (Centrosomal protein C10orf90 homolog) (E2/E3 hybrid ubiquitin-protein ligase FATS) (Fragile-site associated tumor suppressor homolog) (FATS),FUNCTION: Tumor suppressor that is required to sustain G2/M checkpoint after DNA damage (PubMed:20843368, PubMed:20154723, PubMed:24240685). Acts as a p53/TP53 activator by inhibiting MDM2 binding to p53/TP53 and stimulating non-proteolytic polyubiquitination of p53/TP53. Exhibits ubiquitin ligase (E3) activity and assemble ubiquitin polymers through 'Lys-11'- (K11-), 'Lys-29'- (K29-) and 'Lys-63'- (K63)-linkages, independently of the ubiquitin-conjugating enzyme (E2). Promotes p53/TP53-dependent transcription of CDKN1A/p21, leading to robust checkpoint response (PubMed:24240685). Mediates CDKN1A/p21 protein stability in a ubiquitin-independent manner. Interacts with HDAC1 and prevents binding of HDAC1 to CDKN1A/p21 and facilitates the acetylation and stabilization of CDKN1A/p21 (PubMed:20154723). May have a role in the assembly of primary cilia (By similarity). {ECO:0000250|UniProtKB:Q96M02, ECO:0000269|PubMed:20154723, ECO:0000269|PubMed:20843368, ECO:0000269|PubMed:24240685}.
    Molekulargewicht
    71.5 kDa
    UniProt
    D2J0Y4
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