ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore with 1 mL deionized water
Konzentration
1.0 mg/mL (prior to lyophilization)
Buffer
0.01 M phosphate, pH 7.4 containing 0.9 % Sodium Chloride
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-80 °C
Informationen zur Lagerung
Prior to reconstitution store at 2-8 °C. After reconstitution store the antibody at -80 °C. If aliquoted for long term storage, fill volume should be equal to or greater than 50% of the nominal fill volume of the vial used.
Target
Enterotoxin E
Hintergrund
Staphylococcal enterotoxins represent a group of proteins, which are secreted by Staphylococcus aureus and cause the intoxication staphylococcal food poisoning syndrome. The illness characterised by high fever, hypotension, diarrhea, shock, and in some cases death. Their molecular masses range between 27 and 30 kDa. At present, seven enterotoxins are known, namely A, B, C1, C2, C3, D and E. Their amino acid sequences have been determined and it was shown that all are single chain polypeptides containing one disulfide bond formed by two half cystines located in the middle of the polypeptide chain, which form the so called cysteine loop. Enterotoxins are extremely potent activator of T cells, stimulating the production and secretion of various cytokines which mediate many of the toxic effects of these substances. Enterotoxins are Superantigens, inducing polyclonal T cell activation by binding to the TCR and to the alpha chain of the MHC II molecule simultaneously.Synonyms: S. aureus, SEE, entE