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GOGAT Antikörper

GOGAT Reaktivität: Arabidopsis thaliana, Green bean, Miscanthus giganteus, Mais (Zea mays) WB Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN3155732
  • Target
    GOGAT (Glutamine Oxoglutarate Aminotransferase (GOGAT))
    Reaktivität
    Arabidopsis thaliana, Green bean, Miscanthus giganteus, Mais (Zea mays)
    Wirt
    Kaninchen
    Klonalität
    Polyklonal
    Applikation
    Western Blotting (WB)
    Produktmerkmale
    Expected / apparent Molecular Weight of the Antigene: 177 / 170-180 kDa depending upon the species
    This antibody can be used as a plastidial marker
    Aufreinigung
    serum
    Immunogen
    KLH-conjugated synthetic peptide well conserved in known GOGAT sequences from different species including Arabidopsis thaliana Fd-GOGAT 1 Q9ZNZ7, At5g04140 and Fd-GOGAT 2 Q9T0P4, At5g41220
  • Applikationshinweise
    Recommended Dilution: 1 : 1000 with standard ECL (WB) MW 177 | 170 - 180 kDa depending upon the species.
    Kommentare

    A 40 kDa band present in A. thaliana sample is not competed away during antibody neutralization assay. In this assay free peptide used for antibody production is incubated together with anti-GOGAT antibodies. Due to the MW of this protein we suggest to use a gradient gel for protein separation and a longer transfer time.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    For reconstitution add 200 µL of sterile water.
    Handhabung
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    Lagerung
    -20 °C
  • Target
    GOGAT (Glutamine Oxoglutarate Aminotransferase (GOGAT))
    Hintergrund
    AGI Code: At2g41220
    Glutamine oxoglutarate aminotransferase (abbreviated as GOGAT) is an enzyme involved in synthesis of glutamate from glutamine and alpha-ketoglutarate. GOGAT has two forms in plants: ferredoxin-dependent GOGAT (Fd-GOGAT) and NADH-dependent GOGAT (NADH-GOGAT). 95 % of GOGAT found in plants is the Fd-GOGAT type. Fd-GOGAT is encoded by two genes, glu1 and glu2 found on chromosomes 5 and 2 respectively (in Arabidopsis). Fd-GOGAT (both forms) is highly conserved among plants, red algae, and cyanobacteria.
    Molekulargewicht
    expected: 177 kDa, apparent: 170-180 kDa depending upon the species
    UniProt
    Q9T0P4
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