This antibody is reactive with Hsp65 (GroEL) of M. tuberculosis. Does not react with M. bovis, M. avium, M. phlei, M. parafortuitum, Rhodococcus sp., B. subtilis, S. pneumoniae, and E. coli.
Produktmerkmale
Synonyms: 60 kDa chaperonin 2, Protein Cpn60-2, groEL protein 2, Cell wall protein A, Antigen A,groL2, groEL-2, groEL2, hsp65, Rv0440, MT0456, MTV037.04
Aufreinigung
Protein A chromatography
Reinheit
> 90 % pure
Immunogen
Purified protein derivative (PPD)
Klon
BDI578
Isotyp
IgG2a
Applikationshinweise
ELISA. Western Blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0,1 mg/mL (OD280nm, E0.1% = 1.4)
Buffer
0.01 M PBS, pH 7.2 containing 0.09 % sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Informationen zur Lagerung
Store the antibody at -20 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Haltbarkeit
12 months
Target
Heat Shock Protein 65 (HSP65)
Andere Bezeichnung
Heat Shock Protein 65 / HSP65
Synonyme
CPN60 antikoerper, GROEL antikoerper, HLD4 antikoerper, HSP-60 antikoerper, HSP60 antikoerper, HSP65 antikoerper, HuCHA60 antikoerper, SPG13 antikoerper, heat shock protein family D (Hsp60) member 1 antikoerper, HSPD1 antikoerper
Hintergrund
Mycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure, most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method.Synonyms: 60 kDa chaperonin 2, Antigen A, Cell wall protein A, MT0456, MTV037.04, Protein Cpn60-2, Rv0440, groEL protein 2, groEL-2, groEL2, groL2, hsp65