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HSP18.5 Antikörper

HSP18.5-C Reaktivität: Arabidopsis thaliana WB Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN619791
  • Target
    HSP18.5 (HSP18.5-C) (18.5 kDa Class I Heat Shock Protein (HSP18.5-C))
    Reaktivität
    Arabidopsis thaliana
    Wirt
    Kaninchen
    Klonalität
    Polyklonal
    Applikation
    Western Blotting (WB)
    Kreuzreaktivität (Details)
    Not reactive in: no confirmed exceptions from predicted reactivity are currently known
    Homologie
    dicots including: Glycine max, Medicago truncatula, Medicago sativa, Pisum sativum, Ricinus communis, Rosa chinensis, monocots including: Zea mays
    Produktmerkmale
    Expected / apparent Molecular Weight of the Antigene: 18.5 kDa
    Aufreinigung
    serum
    Immunogen
    recombinant Arabidopsis thaliana Hsp18.5
  • Applikationshinweise
    1 : 1000 with standard ECL (WB)
    Kommentare

    hsp 18.5 is a low abundancy protein and estimated concentration of this protein in total cell extract is ca. 0.007-0.01 %. Therefore to be able to visualize this protein the load per well needs to be at least 20 ug of heat shocked total protein/well. It is crucial to heat treat the plants at 38°C for 3 hours at high humidity.Please, note that longer transfer time might result in losing a signal for this protein.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    For reconstitution add 200 μL of sterile water.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Target
    HSP18.5 (HSP18.5-C) (18.5 kDa Class I Heat Shock Protein (HSP18.5-C))
    Andere Bezeichnung
    HSP18.5
    Hintergrund
    HSP18.5 is a small heat shock protein localized in the cytoplasm. The protein belongs to HSP20 family. Synonymes:AtHsp18.5, 18.5 kDa heat shock protein
    Molekulargewicht
    18.5 kDa
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