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Maus IgG1 isotype control (APC)

Name: Maus IgG1 isotype control (APC)
Brand: antibodies-online
SKU: ABIN1027700
Price: 384.05 EUR
Availability: InStock

FACS Monoclonal MOPC-21 IgG1 kappa APC

Flow cytometry: Example of nonspecific mouse IgG1 (MOPC-21) APC signal on human peripheral blood, surface staining, 3 μg/mL. (Maus IgG1 isotype control (APC))

FACS

1 image

(5 Referenzen)

Produktnummer ABIN1027700

Datenblatt (PDF)

Target Alle IgG1 Produkte

IgG1
Wirt
215

41

27

9

4

2

2

1

1

1
Maus
Klonalität
180

11

6
Monoklonal

Konjugat

APC

Applikation

Flow Cytometry (FACS)

Spezifität

This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) with unknown specificity has been confirmed as a good negative control with human and rat species, based on multiple testing on rat and human tissues.

Keine Kreuzreaktivität

Human, Ratte

Aufreinigung

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Klon
43

29

22

12

9

7

6

5

5

4

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
MOPC-21

Isotyp

IgG1 kappa
Mouse IgG1 isotype control (Biotin)
FACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-21 IgG1 kappa Biotin

Mouse IgG1 isotype control (FITC)
FACS, ELISA Monoclonal 15H6 IgG1 FITC

Mouse IgG1 isotype control (PE)
FACS, ELISA Monoclonal 15H6 IgG1 PE

Mouse IgG1 isotype control
FACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p), Func Monoclonal MOPC-21 IgG1 kappa unconjugated

Mouse IgG1 isotype control
FACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-21 IgG1 kappa unconjugated

Mouse IgG1 isotype control (SPRD)
FACS, ELISA Monoclonal 15H6 IgG1 SPRD

Mouse IgG1 isotype control (APC)
FACS, ELISA Monoclonal 15H6 IgG1 APC

Mouse IgG1 isotype control (Biotin)
FACS, ELISA Monoclonal 15H6 IgG1 Biotin

Mouse IgG1 isotype control (PE-Cy7)
FACS, ELISA Monoclonal 15H6 IgG1 PE-Cy7

Mouse IgG1 isotype control (APC-Cy7)
FACS, ELISA Monoclonal 15H6 IgG1 APC-Cy7

Applikationshinweise

Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.

Kommentare

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

0.1 mg/mL

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Do not freeze.
Avoid prolonged exposure to light.

Lagerung

4 °C

Informationen zur Lagerung

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Rebetz, Tian, Persson, Widegren, Salford, Englund, Gisselsson, Fan: "Glial progenitor-like phenotype in low-grade glioma and enhanced CD133-expression and neuronal lineage differentiation potential in high-grade glioma." in: PLoS ONE, Vol. 3, Issue 4, pp. e1936, (2008) (PubMed).

Smed-Sörensen, Moll, Cheng, Loré, Norlin, Perbeck, Moody, Spetz, Sandberg: "IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa." in: Blood, Vol. 111, Issue 10, pp. 5037-46, (2008) (PubMed).

Yates, Rovis, Mitchell, Afzali, Tsang, Garin, Lechler, Lombardi, Garden: "The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro." in: International immunology, Vol. 19, Issue 6, pp. 785-99, (2007) (PubMed).

Carlsten, Björkström, Norell, Bryceson, van Hall, Baumann, Hanson, Schedvins, Kiessling, Ljunggren, Malmberg: "DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells." in: Cancer research, Vol. 67, Issue 3, pp. 1317-25, (2007) (PubMed).

Bryceson, March, Barber, Ljunggren, Long: "Cytolytic granule polarization and degranulation controlled by different receptors in resting NK cells." in: The Journal of experimental medicine, Vol. 202, Issue 7, pp. 1001-12, (2005) (PubMed).
Target

IgG1

Abstract

IgG1 Produkte

Synonyme

IgG1 Isotype Control, Igh-4 Isotype Control, VH7183 Isotype Control, immunoglobulin heavy constant gamma 1 (G1m marker) Isotype Control, Ighg1 Isotype Control

Substanzklasse

Antibody